Comparative biochemical properties of vertebrate deoxyribonuclease I.

Abstract

Deoxyribonuclease I (DNase I, EC 3.1.21.1) is an endonuclease that preferentially attacks double-stranded DNA in a Ca(2+)-dependent manner to produce oligonucleotides with 5'-phospho and 3'-hydroxy termini. This review deals with the biochemical properties and molecular evolution of DNase I. A comparative study of vertebrate DNase I from Chondrichthyes to Homo sapiens has been carried out. The optimal pH, the role of N-glycosylation, actin inhibition, thermal stability, pH stability, and structure stability are discussed. Moreover, a phylogenetic analysis was performed. The levels of DNase I activity in serum have been suggested to be a critical factor in the initiation of human and mouse SLE. Moreover, as shown above, DNase I is utilized in the treatment of patients with cystic fibrosis. Our comparative study of the biochemical properties and molecular analysis of DNase I will be helpful in the use of DNase I for clinical use.

• Authors: Junko Fujihara, Toshihiro Yasuda, Misuzu Ueki, Reiko Iida, Haruo Takeshita
• Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (Comp Biochem Physiol B Biochem Mol Biol) 2012 Nov-Dec Vol. 163 Issue 3-4 Pg. 263-73 ISSN: 1879-1107 [Electronic] England
• PMID: 22885404 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Review)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Deoxyribonuclease I

Topics

• Amino Acid Sequence
• Animals
• Deoxyribonuclease I (chemistry, genetics, metabolism)
• Evolution, Molecular
• Humans
• Molecular Sequence Data
• Mutation
• Vertebrates