Pulmonary impact of N-acetylcysteine in a controlled hemorrhagic shock model in rats.

Abstract

BACKGROUND:

Experimental hemorrhagic shock (HS) is based on controlling bleeding and the treatment of fluid resuscitation to restore tissue oxygenation and perfusion. The HS could promote ischemia/reperfusion injury, which induces a general exacerbation of the inflammatory process, initially compromising the lungs. N-acetylcysteine (NAC), an antioxidant, may attenuate ischemia/reperfusion injury. This study evaluated the effect of NAC in association with fluid resuscitation on pulmonary injury in a controlled HS model in rats.

METHODS:

Male Wistar rats were submitted to controlled HS (mean arterial pressure of 35 mm Hg for 60 min). Two groups were constituted according to resuscitation solution administered: RLG (Ringer's lactate solution) and RLG+NAC (Ringer's lactate in association with 150 mg/kg NAC. A control group was submitted to catheterization only. After 120 min of resuscitation, bronchoalveolar lavage was performed to assess intra-alveolar cell infiltration and pulmonary tissue was collected for assessment of malondialdehyde, interleukin 6, and interleukin 10 and histopathology.

RESULTS:

Compared with the RLG group, the RLG+NAC group showed lower bronchoalveolar lavage inflammatory cell numbers, lower interstitial inflammatory infiltration in pulmonary parenchyma, and lower malondialdehyde concentration. However, tissue cytokine (interleukin 6 and interleukin 10) expression levels were similar.

CONCLUSION:

N-acetylcysteine was associated with fluid resuscitation-attenuated oxidative stress and inflammatory cell infiltration in pulmonary parenchyma. N-acetylcysteine did not modify cytokine expression.

• Authors: Karen Ruggeri Saad, Paulo Fernandes Saad, Luiz Dantas Filho, Jôse Mára de Brito, Marcia Kiyomi Koike, Fernando Luiz Zanoni, Marisa Dolhnikoff, Edna Frasson de Souza Montero
• Journal: The Journal of surgical research (J Surg Res) Vol. 182 Issue 1 Pg. 108-15 (Jun 01 2013) ISSN: 1095-8673 [Electronic] United States
• PMID: 22883437 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Interleukin-6
• Interleukin-10
• Malondialdehyde
• Acetylcysteine

Topics

• Acetylcysteine (pharmacology, therapeutic use)
• Acute Lung Injury (metabolism, pathology, prevention & control)
• Animals
• Antioxidants (pharmacology, therapeutic use)
• Disease Models, Animal
• Fluid Therapy
• Interleukin-10 (metabolism)
• Interleukin-6 (metabolism)
• Lung (drug effects, metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Resuscitation
• Shock, Hemorrhagic (complications)