Anti-inflammatory effects of oleanolic acid on LPS-induced inflammation in vitro and in vivo.

Abstract

Oleanolic acid (OA) is a triterpenoid known for its anti-inflammatory and anti-cancer properties; however, the anti-inflammatory effects of OA on lipopolysaccharide (LPS)-mediated pro-inflammatory responses have not been studied. Here, we first investigated the possible anti-inflammatory effects of OA against pro-inflammatory responses in human umbilical vein endothelial cells (HUVECs) induced by LPS and the associated signaling pathways. We found that OA inhibited LPS-induced barrier disruption, expression of cell adhesion molecules (CAMs), and adhesion/transendothelial migration of monocytes to HUVECs. OA also suppressed acetic acid-induced hyperpermeability and carboxymethylcellulose-induced leukocyte migration in vivo. Further studies revealed that OA suppressed the production of tumor necrosis factor-α and activation of nuclear factor-κB by LPS. Collectively, these results suggest that OA has anti-inflammatory effects by inhibiting hyperpermeability, the expression of CAMs, and the adhesion and migration of leukocytes, thereby endorsing its usefulness as a therapeutic agent for vascular inflammatory diseases.

• Authors: Wonhwa Lee, Eun-Ju Yang, Sae-Kwang Ku, Kyung-Sik Song, Jong-Sup Bae
• Journal: Inflammation (Inflammation) Vol. 36 Issue 1 Pg. 94-102 (Feb 2013) ISSN: 1573-2576 [Electronic] United States
• PMID: 22875543 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• E-Selectin
• Lipopolysaccharides
• NF-kappa B
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• Intercellular Adhesion Molecule-1
• Oleanolic Acid
• Carboxymethylcellulose Sodium
• Acetic Acid

Topics

• Acetic Acid
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
• Carboxymethylcellulose Sodium
• Cell Adhesion (drug effects)
• Cell Line
• Cell Movement (drug effects)
• Cell Survival
• E-Selectin
• Endothelium, Vascular (cytology, drug effects, metabolism)
• Enzyme Activation (drug effects)
• Female
• Human Umbilical Vein Endothelial Cells
• Humans
• Inflammation (chemically induced, drug therapy)
• Intercellular Adhesion Molecule-1 (metabolism)
• Lipopolysaccharides
• Mice
• Mice, Inbred ICR
• NF-kappa B (metabolism)
• Oleanolic Acid (pharmacology)
• Transendothelial and Transepithelial Migration (drug effects)
• Tumor Necrosis Factor-alpha (biosynthesis)
• Vascular Cell Adhesion Molecule-1 (metabolism)