Transcriptional and translational plasticity in rodent urinary bladder TRP channels with urinary bladder inflammation, bladder dysfunction, or postnatal maturation.

Abstract

These studies examined the transcriptional and translational plasticity of three transient receptor potential (TRP) channels (TRPA1, TRPV1, TRPV4) with established neuronal and non-neuronal expression and functional roles in the lower urinary tract. Mechanosensor and nociceptor roles in either physiological or pathological lower urinary tract states have been suggested for TRPA1, TRPV1, and TRPV4. We have previously demonstrated the neurochemical, organizational, and functional plasticity in micturition reflex pathways following induction of urinary bladder inflammation using the antineoplastic agent, cyclophosphamide. More recently, we have characterized similar plasticity in micturition reflex pathways in a transgenic mouse model with chronic urothelial overexpression (OE) of nerve growth factor (NGF) and in a transgenic mouse model with deletion of vasoactive intestinal polypeptide (VIP). In addition, the micturition reflex undergoes postnatal maturation that may also reflect plasticity in urinary bladder TRP channel expression. Thus, we examined plasticity in urinary bladder TRP channel expression in diverse contexts using a combination of quantitative, real-time PCR and western blotting approaches. We demonstrate transcriptional and translational plasticity of urinary bladder TRPA1, TRPV1, and TRVP4 expression. Although the functional significance of urinary bladder TRP channel plasticity awaits further investigation, these studies demonstrate context- (inflammation, postnatal development, NGF-OE, VIP deletion) and tissue-dependent (urothelium + suburothelium, detrusor) plasticity.

• Authors: Liana Merrill, Beatrice M Girard, Victor May, Margaret A Vizzard
• Journal: Journal of molecular neuroscience : MN (J Mol Neurosci) Vol. 48 Issue 3 Pg. 744-56 (Nov 2012) ISSN: 1559-1166 [Electronic] United States
• PMID: 22865090 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Recombinant Fusion Proteins
• Vasoactive Intestinal Peptide
• Cyclophosphamide
• Nerve Growth Factor

Topics

• Aging (physiology)
• Animals
• Cyclophosphamide (toxicity)
• Cystitis (chemically induced, genetics, metabolism, physiopathology)
• Disease Progression
• Female
• Gene Expression Regulation (physiology)
• Mice
• Mice, Knockout
• Mice, Transgenic
• Muscle, Smooth (metabolism)
• Nerve Growth Factor (biosynthesis, genetics, physiology)
• Organ Specificity
• Protein Biosynthesis
• Rats
• Rats, Wistar
• Recombinant Fusion Proteins (biosynthesis, physiology)
• Reflex, Abnormal
• Transcription, Genetic
• Urinary Bladder (growth & development, metabolism, physiopathology)
• Urination (physiology)
• Urothelium (metabolism)
• Vasoactive Intestinal Peptide (deficiency, genetics)