Mast cell degranulation mediates compound 48/80-induced hyperalgesia in mice.

Abstract

Mast cells mediate allergies, hypersensitivities, host defense, and venom neutralization. An area of recent interest is the contribution of mast cells to inflammatory pain. Here we found that specific, local activation of mast cells produced plantar hyperalgesia in mice. Basic secretagogue compound 48/80 induced plantar mast cell degranulation accompanied by thermal hyperalgesia, tissue edema, and neutrophil influx in the hindpaws of ND4 Swiss mice. Blocking mast cell degranulation, neutrophil extravasation, and histamine signaling abrogated these responses. Compound 48/80 also produced edema, pain, and neutrophil influx in WT C57BL/6 but not in genetically mast cell-deficient C57BL/6-Kit(W-sh)(/)(W-sh) mice. These responses were restored following plantar reconstitution with bone marrow-derived cultured mast cells.

• Authors: Devavani Chatterjea, Abigail Wetzel, Madison Mack, Camilla Engblom, Juliann Allen, Carolina Mora-Solano, Luisa Paredes, Evelyn Balsells, Tijana Martinov
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 425 Issue 2 Pg. 237-43 (Aug 24 2012) ISSN: 1090-2104 [Electronic] United States
• PMID: 22828511 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• p-Methoxy-N-methylphenethylamine

Topics

• Animals
• Cell Degranulation
• Edema (chemically induced, immunology)
• Hot Temperature
• Hyperalgesia (chemically induced, immunology)
• Male
• Mast Cells (drug effects, immunology)
• Mice
• Mice, Inbred C57BL
• Mice, Mutant Strains
• Neutrophil Infiltration (drug effects, immunology)
• Neutrophils (drug effects, immunology)
• p-Methoxy-N-methylphenethylamine (pharmacology)