Abstract | PURPOSE: EXPERIMENTAL DESIGN: RBC membrane proteins of HE, HS, and control groups were compared by 2DE and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS analysis was obtained using peptide mass fingerprint for protein identification. RESULTS: CONCLUSIONS AND CLINICAL RELEVANCE: 2DE of RBC proteins is a potentially valuable method for studying heritable disorders such as HE and HS. By identifying a deficiency in membrane proteins associated with the RBC cytoskeleton, the diagnosis of HE and HS can be confirmed.
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Authors | Duygu Ozel Demiralp, Selen Peker, Burhan Turgut, Nejat Akar |
Journal | Proteomics. Clinical applications
(Proteomics Clin Appl)
Vol. 6
Issue 7-8
Pg. 403-11
(Aug 2012)
ISSN: 1862-8354 [Electronic] Germany |
PMID | 22807418
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
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Topics |
- Case-Control Studies
- Electrophoresis, Gel, Two-Dimensional
(methods)
- Elliptocytosis, Hereditary
(metabolism)
- Erythrocyte Membrane
(metabolism)
- Humans
- Membrane Proteins
(deficiency, metabolism)
- Proteomics
(methods)
- Spherocytosis, Hereditary
(metabolism)
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