Abstract | BACKGROUND: OBJECTIVE: In this study, we constructed a CAP-FEIA with recombinant HMW- glutenin, and evaluated its usefulness in identifying the patients with WDEIA. METHODS: RESULTS: In 16 AD patients without wheat allergy 12 of them (75%) had positive results for native HMW- glutenin test in contrast to epitope peptide of HMW- glutenin (12.5%) and recombinant HMW- glutenin test (12.5%). These results indicate the native HMW- glutenin test has low specificity. Sensitivity and specificity of the IgE test with recombinant HMW- glutenin were 16.7% and 92.9%. These are well compatible with results obtained by using epitope peptide of HMW- glutenin. However, sensitivity and specificity reached to 93.8% and 92.9%, when the test was combined to the test with recombinant omega-5 gliadin. CONCLUSIONS AND CLINICAL RELEVANCE: We demonstrated that recombinant HMW- glutenin is best for CAP-FEIA system in point of stability and specificity and confirmed that detection of specific IgE against recombinant HMW- glutenin is useful for diagnosis of WDEIA when combined with the CAP-FEIA (recombinant omega-5 gliadin) test.
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Authors | H Takahashi, H Matsuo, Y Chinuki, K Kohno, A Tanaka, N Maruyama, E Morita |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 42
Issue 8
Pg. 1293-8
(Aug 2012)
ISSN: 1365-2222 [Electronic] England |
PMID | 22805477
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2012 Blackwell Publishing Ltd. |
Chemical References |
- Antigens, Plant
- Protein Subunits
- Recombinant Proteins
- omega-5 gliadin, wheat
- Immunoglobulin E
- Glutens
- Gliadin
- glutenin
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Topics |
- Anaphylaxis
(diagnosis, immunology)
- Antibody Specificity
(immunology)
- Antigens, Plant
(immunology)
- Exercise
- Gliadin
(immunology)
- Glutens
(chemistry, immunology)
- Humans
- Immunoglobulin E
(blood, immunology, metabolism)
- Molecular Weight
- Protein Binding
(immunology)
- Protein Subunits
(immunology, metabolism)
- Recombinant Proteins
(immunology, metabolism)
- Wheat Hypersensitivity
(diagnosis, immunology)
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