Abstract | PURPOSE: METHODS: The in vitro susceptibility of AMB and AME was determined for C. albicans isolates recovered from endophthalmitis (N=10) and for C. albicans ATCC reference strain 90028 using the Clinical and Laboratory Standards Institute M27-A2 (NCCLS/CLSI) broth dilution method. All isolates were obtained from samples of vitreous humor of patients with suspected endophthalmitis within the last 5 years at the Bascom Palmer Eye Institute, University of Miami Miller School of Medicine (Miami, FL). RESULTS: The minimal inhibitory concentrations (MICs) of AME were equal to or lower than values for AMB in 7 of the 10 isolates; range: AME (0.125-1 μg/mL) versus (0.5-1 μg/mL) for AMB. The MIC(90) value of both drugs was equal (1 μg/mL). Compared with AMB, the minimal fungicidal concentrations (MFCs) of AME were equal to or lower in 8 of 10 isolates; range: AME (0.125-2 μg/mL) versus AMB (0.25-4 μg/mL). MFC(90) values of AME (1 μg/mL) was slightly superior to AMB (2 μg/mL). The MIC of the quality control strain (ATCC(®) 90028) was within an acceptable range. CONCLUSIONS:
AME was equivalent to AMB in vitro against C. albicans. This formula may offer a slightly more efficient and less toxic formulation for the treatment of Candida endophthalmitis.
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Authors | Onsiri Thanathanee, Darlene Miller, David M Ringel, Carl P Schaffner, Eduardo C Alfonso, Terrence P O'Brien |
Journal | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics
(J Ocul Pharmacol Ther)
Vol. 28
Issue 6
Pg. 589-92
(Dec 2012)
ISSN: 1557-7732 [Electronic] United States |
PMID | 22788845
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antifungal Agents
- methylamphotericin B
- Amphotericin B
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Topics |
- Amphotericin B
(adverse effects, analogs & derivatives, chemistry, pharmacology)
- Antifungal Agents
(adverse effects, chemistry, pharmacology)
- Candida albicans
(drug effects, isolation & purification)
- Candidiasis
(drug therapy, microbiology)
- Endophthalmitis
(microbiology)
- Eye Infections, Fungal
(microbiology)
- Humans
- Microbial Sensitivity Tests
- Solubility
- Vitreous Body
(microbiology)
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