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Comparative in vitro antifungal susceptibility activity of amphotericin B versus amphotericin B methyl ester against Candida albicans ocular isolates.

AbstractPURPOSE:
To compare in vitro susceptibility of amphotericin B (AMB) and amphotericin B methyl ester (AME) (a more soluble and less toxic formulation of AMB) against Candida albicans isolates recovered from human cases of endophthalmitis.
METHODS:
The in vitro susceptibility of AMB and AME was determined for C. albicans isolates recovered from endophthalmitis (N=10) and for C. albicans ATCC reference strain 90028 using the Clinical and Laboratory Standards Institute M27-A2 (NCCLS/CLSI) broth dilution method. All isolates were obtained from samples of vitreous humor of patients with suspected endophthalmitis within the last 5 years at the Bascom Palmer Eye Institute, University of Miami Miller School of Medicine (Miami, FL).
RESULTS:
The minimal inhibitory concentrations (MICs) of AME were equal to or lower than values for AMB in 7 of the 10 isolates; range: AME (0.125-1 μg/mL) versus (0.5-1 μg/mL) for AMB. The MIC(90) value of both drugs was equal (1 μg/mL). Compared with AMB, the minimal fungicidal concentrations (MFCs) of AME were equal to or lower in 8 of 10 isolates; range: AME (0.125-2 μg/mL) versus AMB (0.25-4 μg/mL). MFC(90) values of AME (1 μg/mL) was slightly superior to AMB (2 μg/mL). The MIC of the quality control strain (ATCC(®) 90028) was within an acceptable range.
CONCLUSIONS:
AME was equivalent to AMB in vitro against C. albicans. This formula may offer a slightly more efficient and less toxic formulation for the treatment of Candida endophthalmitis.
AuthorsOnsiri Thanathanee, Darlene Miller, David M Ringel, Carl P Schaffner, Eduardo C Alfonso, Terrence P O'Brien
JournalJournal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics (J Ocul Pharmacol Ther) Vol. 28 Issue 6 Pg. 589-92 (Dec 2012) ISSN: 1557-7732 [Electronic] United States
PMID22788845 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Antifungal Agents
  • methylamphotericin B
  • Amphotericin B
Topics
  • Amphotericin B (adverse effects, analogs & derivatives, chemistry, pharmacology)
  • Antifungal Agents (adverse effects, chemistry, pharmacology)
  • Candida albicans (drug effects, isolation & purification)
  • Candidiasis (drug therapy, microbiology)
  • Endophthalmitis (microbiology)
  • Eye Infections, Fungal (microbiology)
  • Humans
  • Microbial Sensitivity Tests
  • Solubility
  • Vitreous Body (microbiology)

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