Tyrosinase catalyzes in mammals the first and rate-limiting step in the biosynthesis of the
melanin, the main pigment of the skin.
Pterins,
heterocyclic compounds able to photoinduce oxidation of
DNA and its components, accumulate in the skin of patients suffering from
vitiligo, a chronic depigmentation disorder in which the protection against UV radiation fails due to the lack of
melanin. Aqueous solutions of
tyrosinase were exposed to UV-A irradiation (350 nm) in the presence of
pterin, the parent compound of oxidized
pterins, under different experimental conditions. The
enzyme activity in the irradiated solutions was determined by spectrophotometry and HPLC. In this work, we present data that demonstrate unequivocally that the
enzyme is photoinactivated by
pterin. The mechanism of the photosensitized process involves an electron transfer from
tyrosinase to the triplet excited state of
pterin, formed after UV-A excitation of
pterin. The
biological implications of the results are discussed.