Abstract | OBJECTIVE: METHODS: The carbodiimide crosslinking method was used to couple protein G and goat antihuman IgG on the surface of water-solubility QDs. The coverglass covered HV antigen was used as carrier, and QDs-PG- IgG conjugates was used as labeled second antibody to detect the HV- IgG in the serum samples. The detecting conditions were optimized. RESULTS: The optimum reaction time, pH and goat antihuman IgG concentration for conjugating the QDs with goat antihuman IgG were 6.0, 2h, and 20 microg/ml, respectively. The optimum working dilution of QDs-PG- IgG conjugates was 1: 200. The detection limit of the serum samples was about 1: 1280 dilution. CONCLUSION: The method established in this study has been demonstrated to be a specific, sensitive, rapid test for detecting HV antibodies, laying the foundation of single molecule detection. The anti-fluorescence quenching ability of this method was significant improved.
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Authors | Ya Shen, Jie Wang, Long Ba, Ping-Ping Yao, Xiao-Zhao Deng, Chang-Jun Wang, Han-Ping Zhu, Yun Zhang, Zhan-Qiu Yang |
Journal | Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
(Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi)
Vol. 25
Issue 6
Pg. 486-8
(Dec 2011)
ISSN: 1003-9279 [Print] China |
PMID | 22734244
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Immunoglobulin G
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Topics |
- Antibodies, Viral
(blood)
- Fluorescence
- Hantavirus Infections
(diagnosis)
- Humans
- Immunoassay
(methods)
- Immunoglobulin G
(blood)
- Quantum Dots
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