[A study of immunoassay by using quantum dots to detect Hantavirus infection].

Abstract

OBJECTIVE:

To develop a new method to detect anti-Hantavirus IgG antibodies (HV IgG) based on quantum dots (QDs) and indirect immune technique.

METHODS:

The carbodiimide crosslinking method was used to couple protein G and goat antihuman IgG on the surface of water-solubility QDs. The coverglass covered HV antigen was used as carrier, and QDs-PG-IgG conjugates was used as labeled second antibody to detect the HV-IgG in the serum samples. The detecting conditions were optimized.

RESULTS:

The optimum reaction time, pH and goat antihuman IgG concentration for conjugating the QDs with goat antihuman IgG were 6.0, 2h, and 20 microg/ml, respectively. The optimum working dilution of QDs-PG-IgG conjugates was 1: 200. The detection limit of the serum samples was about 1: 1280 dilution.

CONCLUSION:

The method established in this study has been demonstrated to be a specific, sensitive, rapid test for detecting HV antibodies, laying the foundation of single molecule detection. The anti-fluorescence quenching ability of this method was significant improved.

• Authors: Ya Shen, Jie Wang, Long Ba, Ping-Ping Yao, Xiao-Zhao Deng, Chang-Jun Wang, Han-Ping Zhu, Yun Zhang, Zhan-Qiu Yang
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 25 Issue 6 Pg. 486-8 (Dec 2011) ISSN: 1003-9279 [Print] China
• PMID: 22734244 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin G

Topics

• Antibodies, Viral (blood)
• Fluorescence
• Hantavirus Infections (diagnosis)
• Humans
• Immunoassay (methods)
• Immunoglobulin G (blood)
• Quantum Dots