Distribution of IL-3 activity in mice transplanted with IL-3 producing T cells (STIL-3): abrogation of the distribution of STIL-3 cells into the liver and liver hemopoiesis by splenectomy.

Abstract

Distribution of IL-3 producing T cells, STIL-3, in splenectomized mice after intravenous injection was studied in order to determine the site of IL-3 production in the splenectomized mice. Hematological features of STIL-3-inoculated mice after splenectomy were also studied. Splenectomy prior to inoculation of STIL-3 cells resulted in a slight increase in the level of IL-3 activity in most lymphohemopoietic tissues, but resulted in a slight decrease in the level of IL-3 activity in the plasma. The splenectomy also abrogated granulocytosis which was evident in sham-operated host mice. Hepatomegaly and a number of granulocytic foci in the liver were observed in STIL-3 inoculated mice, but this was again abrogated completely by the splenectomy. These results suggest that the spleen is the major site of granulopoiesis in response to the stimulus with IL-3, and also that the spleen is essential for the migration or metastasis of STIL-3 cells to the liver.

• Authors: K Aramaki, N Takayama, S Taniguchi, M Miyashita, K Sugimoto, K J Mori
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 44 Issue 8 Pg. 429-34 ( 1990) ISSN: 0753-3322 [Print] France
• PMID: 2271741 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-3

Topics

• Animals
• Cell Count
• Female
• Granulocytes (cytology)
• Hematopoiesis
• Interleukin-3 (metabolism)
• Liver (cytology, metabolism)
• Mice
• Mice, Inbred C3H
• Postoperative Period
• Splenectomy
• T-Lymphocytes (cytology, metabolism, transplantation)