Abstract | BACKGROUND: 'Conditioning' [ischemic preconditioning (IPC), ischemic postconditioning (IPO) and remote ischemic preconditioning (RIPC)] the heart to render it more resistant to an episode of acute myocardial ischemia-reperfusion (I/R) injury is an endogenous cardioprotective strategy. There are several mechanisms proposed for 'conditioning', such as endogenous mediators or cytoprotective proteins. In recent reports, microRNAs ( miRNAs) were involved in controlling the expression of myocardial ischemia-related genes. Some studies have demonstrated that cardiac miRNA-1 and miRNA-21 were significantly increased by late IPC with an increase in their target proteins [ endothelial nitric oxide synthase and heat shock protein 70 (HSP70)], but their expression levels in 'conditioning' strategies are currently unknown. METHODS: RESULTS: The results revealed that miRNA-1 (233 ± 45%) and miRNA-21 (356 ± 33%) expression was up-regulated in the IPC group, but the expression of miRNA-1 was down-regulated in the RIPC (61 ± 16%) group and IPO group (61 ± 13%). The expression of PDCD4 [IPC (74 ± 11%), RIPC (81 ± 16%), IPO (83 ± 12%)], HSP70 [IPC (74 ± 5%), RIPC (81 ± 6%), IPO (67 ± 11%)] and Bax [IPC (27 ± 6%), RIPC (21 ± 3%), IPO (27 ± 4%)] was down-regulated in the conditioning groups compared with the CON group [PDCD4 (130 ± 11%), HSP70 (121 ± 11%) and Bax (63 ± 8%)]. In the conditioning hearts, infarct size [IPC (31.7 ± 4.1%), RIPC (29.6 ± 6.19%) and IPO (32.8 ± 4.71%)] and myocardial apoptosis [IPC (15.2 ± 4.21%), RIPC (17.2 ± 1.92%) and IPO (15.6 ± 4.04%)] were significantly decreased compared with the CON group ( infarct size: 51.77 ± 4.3%, myocardial apoptosis: 32.8 ± 3.96%). CONCLUSION: We concluded that miRNA-1 and miRNA-21 expression differed in IPC, RIPC and IPO groups, and their target proteins were not inversely correlated with the miRNAs in all the conditioning groups, which revealed that the miRNAs were regulated but complicated by the different conditioning protocols.
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Authors | Xin Duan, Bingyang Ji, Xiaohua Wang, Jinping Liu, Zhe Zheng, Cun Long, Yue Tang, Shengshou Hu |
Journal | Cardiology
(Cardiology)
Vol. 122
Issue 1
Pg. 36-43
( 2012)
ISSN: 1421-9751 [Electronic] Switzerland |
PMID | 22699357
(Publication Type: Journal Article)
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Copyright | Copyright © 2012 S. Karger AG, Basel. |
Chemical References |
- Apoptosis Regulatory Proteins
- HSP70 Heat-Shock Proteins
- MIRN1 microRNA, human
- MIRN21 microRNA, human
- MicroRNAs
- Pdcd4 protein, rat
- Proteins
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
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Topics |
- Animals
- Apoptosis
- Apoptosis Regulatory Proteins
(metabolism)
- Blotting, Western
- Disease Models, Animal
- Down-Regulation
- HSP70 Heat-Shock Proteins
(metabolism)
- Hemodynamics
(physiology)
- In Vitro Techniques
- Ischemic Preconditioning, Myocardial
(methods)
- Ligation
- Male
- MicroRNAs
(metabolism)
- Myocardial Infarction
- Myocardial Ischemia
(metabolism)
- Proteins
(metabolism)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Up-Regulation
- bcl-2-Associated X Protein
(metabolism)
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