Hepatic nitrosative stress in experimental diabetes.

Abstract

AIM:

The effects of the inhibition of nitrosative stress by aminoguanidine in an experimental model of diabetes mellitus (DM) were investigated.

METHODS:

Twenty-one male Wistar rats were divided into three groups: control (CO), diabetic (DM), and diabetic treated with aminoguanidine (DM+AG). Aminoguanidine (aminoguanidine hemisulfate salt, Sigma Chemical Co., St. Louis, MO, USA) was used at a dose of 50 mg/kg (i.p.) during the last 30 days of the experiment. The expression levels of liver lipoperoxidation (TBARS - nmol/mg protein), inducible oxide nitric synthase (iNOS), nitrotyrosine and the NFκB nuclear transcription factor p65 were examined using western blot analysis.

RESULTS:

The DM group demonstrated an increase in lipoperoxidation and in the expression of iNOS, nitrotyrosine and p65. Aminoguanidine reduced hepatic lipid peroxidation and protein expression levels of iNOS, nitrotyrosine and p65.

CONCLUSION:

Aminoguanidine treatment reduces liver oxidative and nitrosative stress in diabetic animals. In addition, aminoguanidine reduced the expression of p65 in the liver.

• Authors: Fábio Cangeri Di Naso, Graziella Rodrigues, Alexandre Simões Dias, Marilene Porawski, Henrique Fillmann, Norma Possa Marroni
• Journal: Journal of diabetes and its complications (J Diabetes Complications) 2012 Sep-Oct Vol. 26 Issue 5 Pg. 378-81 ISSN: 1873-460X [Electronic] United States
• PMID: 22699114 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• Enzyme Inhibitors
• Guanidines
• Reactive Nitrogen Species
• Reactive Oxygen Species
• Rela protein, rat
• Transcription Factor RelA
• 3-nitrotyrosine
• Tyrosine
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type II
• Nos2 protein, rat
• pimagedine

Topics

• Animals
• Diabetes Mellitus, Experimental (drug therapy)
• Down-Regulation (drug effects)
• Enzyme Inhibitors (therapeutic use)
• Guanidines (therapeutic use)
• Lipid Peroxidation (drug effects)
• Liver (drug effects, enzymology, metabolism)
• Male
• Nitric Oxide Synthase (antagonists & inhibitors)
• Nitric Oxide Synthase Type II (metabolism)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Reactive Nitrogen Species (metabolism)
• Reactive Oxygen Species (metabolism)
• Transcription Factor RelA (metabolism)
• Tyrosine (analogs & derivatives, metabolism)