Abstract | OBJECTIVES: METHODS: Ninety-six male Sprague-Dawley rats were randomly divided into four groups ( sham operation group, ANP group, melatonin treatment group, melatonin contrast group). ANP was induced by the retrograde injection of 4% taurocholate (1 ml/kg body weight) into the biliopancreatic duct. Melatonin (50 mg/kg body weight) was administered 30 min before the induction of ANP in the melatonin treatment group. Rats in each group were euthanized at 1, 4, and 8 h after ANP induction. Pancreatic tissues were removed to measure SERCA and NCX levels and cytosolic calcium ion (Ca(2+)) concentration ([Ca(2+)](i)). RESULTS: At each time point, SERCA and NCX levels in the melatonin treatment group were significantly higher than that in the ANP group, and lower than that in the sham group and the melatonin contrast group. These levels did not differ between the 4- and 8-h time points in the ANP group. [Ca(2+)](i) in pancreatic acinar cells was higher in the melatonin treatment group than in the sham group and the melatonin contrast group, but lower than in the ANP group, at each time point. CONCLUSION:
Melatonin can reduce pancreatic damage via the up-regulation of SERCA and NCX expression, which can alleviate calcium overload in pancreatic acinar cells.
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Authors | Jiaping Huai, Yingying Shao, Xuecheng Sun, Yin Jin, Jiansheng Wu, Zhiming Huang |
Journal | Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
(Pancreatology)
2012 May-Jun
Vol. 12
Issue 3
Pg. 257-63
ISSN: 1424-3911 [Electronic] Switzerland |
PMID | 22687382
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 IAP and EPC. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Sodium-Calcium Exchanger
- Sarcoplasmic Reticulum Calcium-Transporting ATPases
- Melatonin
- Calcium
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Topics |
- Animals
- Calcium
(metabolism)
- Cytosol
(metabolism)
- Down-Regulation
- Male
- Melatonin
(therapeutic use)
- Pancreatitis, Acute Necrotizing
(prevention & control)
- Rats
- Rats, Sprague-Dawley
- Sarcoplasmic Reticulum Calcium-Transporting ATPases
(biosynthesis, metabolism)
- Sodium-Calcium Exchanger
(biosynthesis, metabolism)
- Up-Regulation
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