Amphotericin B-induced in vitro postantifungal effect on Candida species of oral origin.

Abstract

OBJECTIVE:

The aim of this investigation was to measure the postantifungal effect (PAFE) of 6 different oral Candida species following exposure to amphotericin B.

MATERIALS AND METHODS:

Five oral isolates each of Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis, Candida glabrata and Candida guilliermondii (total of 30 isolates) were examined for the presence of PAFE after 1 h of exposure to the minimum inhibitory concentration of amphotericin B. The PAFE was determined as the difference in time (hours) required for the growth of the drug-free control and the drug-exposed test cultures to increase to 0.05 absorbance level following removal of amphotericin B.

RESULTS:

The mean duration of amphotericin B-induced PAFE was lowest for C. albicans (5.91 ± 0.31 h) and greatest for C. parapsilosis (12.72 ± 0.11 h), while C. guilliermondii (8.32 ± 0.33 h), C. glabrata (8.43 ± 0.21 h), C. krusei (9.68 ± 0.23 h) and C. tropicalis (10.98 ± 0.18 h) elicited intermediate values.

CONCLUSION:

Even a limited exposure to sublethal concentrations of amphotericin B suppressed growth of Candida species of oral origin. The significant variation in amphotericin B-induced PAFE amongst different Candida species may have clinical implications in terms of amphotericin B regimens used in the management of oral candidiasis.

• Authors: A N B Ellepola
• Journal: Medical principles and practice : international journal of the Kuwait University, Health Science Centre (Med Princ Pract) Vol. 21 Issue 5 Pg. 442-6 ( 2012) ISSN: 1423-0151 [Electronic] Switzerland
• PMID: 22678101 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 S. Karger AG, Basel.

Chemical References

• Antifungal Agents
• Amphotericin B

Topics

• Amphotericin B (pharmacology)
• Antifungal Agents (pharmacology)
• Candida (drug effects, isolation & purification)
• Candidiasis, Oral (microbiology)
• Humans
• Microbial Sensitivity Tests