Abstract | BACKGROUND: METHODS: Rats were randomly divided into five groups: the non-HBO2P + non-HAE group, the HBO2P + non-HAE group, the non-HBO2P + HAE group, the HBO2P + HAE group, and the HBO2P + HSP-70 Abs + HAE group. The HBO2P groups were given 100% O2 at 2.0 absolute atmospheres for 1 hour per day for 5 consecutive days. The HAE groups were exposed to simulated HAE (9.7% O2 at 0.47 absolute atmospheres of 6,000 m) in a hypobaric chamber for 3 days. Polyclonal rabbit anti-mouse HSP-70-neutralizing Abs were intravenously injected 24 hours before the HAE experiments. Immediately after returning to normal atmosphere, the rats were given cognitive performance tests, overdosed with a general anesthetic, and then their brains were excised en bloc for water content measurements and biochemical evaluation and analysis. RESULTS: Non-HBO2P group rats displayed cognitive deficits, brain edema, and hippocampal oxidative stress (evidenced by increased toxic oxidizing radicals [e.g., nitric oxide metabolites and hydroxyl radicals], increased pro-oxidant enzymes [e.g., malondialdehyde and oxidized glutathione] but decreased antioxidant enzymes [e.g., reduced glutathione, glutathione peroxide, glutathione reductase, and superoxide dismutase]) in HAE. HBO2P induced HSP-70 overexpression in the hippocampus and significantly attenuated HAE-induced brain edema, cognitive deficits, and hippocampal oxidative stress. The beneficial effects of HBO2P were significantly reduced by HSP-70 Ab preconditioning. CONCLUSION: Our results suggest that high-altitude cerebral edema, cognitive deficit, and hippocampal oxidative stress can be prevented by HSP-70-mediated HBO2P in rats.
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Authors | Hung Lin, Ching-Ping Chang, Hung-Jung Lin, Mao-Tsun Lin, Cheng-Chia Tsai |
Journal | The journal of trauma and acute care surgery
(J Trauma Acute Care Surg)
Vol. 72
Issue 5
Pg. 1220-7
(May 2012)
ISSN: 2163-0763 [Electronic] United States |
PMID | 22673248
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HSP70 Heat-Shock Proteins
- Nitric Oxide
- Malondialdehyde
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Topics |
- Altitude Sickness
(complications, metabolism, pathology)
- Animals
- Brain Edema
(etiology, metabolism, therapy)
- Cognition Disorders
(etiology, physiopathology, therapy)
- Disease Models, Animal
- HSP70 Heat-Shock Proteins
(biosynthesis)
- Hippocampus
(metabolism, pathology, physiopathology)
- Hyperbaric Oxygenation
(methods)
- Male
- Malondialdehyde
(metabolism)
- Nitric Oxide
(metabolism)
- Oxidative Stress
- Rats
- Rats, Wistar
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