[Influence of Aconiti Lateralis Radix Praeparata on asthenia cold syndrome rats with whole genome gene expression of liver by gene chip technique].

Abstract

OBJECTIVE:

To study the influence of Aconiti Lateralis Radix Praeparata on asthenia cold syndrome rats with whole genome gene expression of liver by gene chip technique.

METHOD:

The asthenia cold syndrome rat models were established by administering traditional Chinese medicine raw Gypsum Fibrosum, Gentianae Radix, Phellodendri Chinensis Cortex and Anemarrhenae Rhizoma. After treated with Aconiti Lateralis Radix Praeparata, the rats' liver gene expressions were detected using gene chip. Differential expression genes were screened for gene function annotation, and some genes were selected to check the accuracy of the results by RT-PCR.

RESULT:

Compared with the asthenia cold model group, the asthenia cold treatment group showed 212 differential expression genes, mainly involving function of immune response and oxidoreductase activity.

CONCLUSION:

Aconiti Lateralis Radix Praeparata is proved to have an effect on up-regulating immune response-related genes and oxidizing oxidoreductase activity-related genes of asthenia cold syndrome rats and may be a molecular mechanism for classical warm-nature medicine Aconiti Lateralis Radix Praeparata in warming meridians and dissipating cold.

• Authors: Bingbing Han, Shijun Wang, Fayan Zhang, Haijun Zhao, Chenggang Wang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 37 Issue 4 Pg. 500-4 (Feb 2012) ISSN: 1001-5302 [Print] China
• PMID: 22667152 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal

Topics

• Aconitum (chemistry)
• Animals
• Asthenia (drug therapy, genetics)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Female
• Gene Expression Profiling
• Genomics
• Liver (drug effects, metabolism)
• Male
• Oligonucleotide Array Sequence Analysis
• Rats
• Rats, Wistar