Abstract | BACKGROUND: METHODS: Seventy-five male Sprague Dawley rats (weighing 300-350 g) were randomly assigned to five groups (n = 15): (1) sham, (2) SAH, (3) SAH + DMSO ( dimethyl sulfoxide), (4) SAH + 10 mg/kg SP600125, and (5) SAH + 30 mg/kg SP600125. SP600125 or DMSO was injected intraperitoneally 1 h before and 6 h after the induction of SAH. Animals from all the groups were killed 24 h after SAH, and brain tissues were dissected and subjected to electron microscopic examination, Western blot analysis, and histological evaluation. RESULTS:
SP600125 pretreatment restored tight junctions and attenuated blood-brain barrier (BBB) disruption and cerebral edema after SAH, coupled with reduced apoptosis in the cerebral cortex. SP600125 exposure restored the reduced expression of both claudin-5 and ZO-1 following SAH and normalized the levels of JNK1 and JNK3. CONCLUSION: Our data demonstrate that the JNK signaling plays an important role in the regulation of tight junction proteins and BBB integrity, and thus represents a promising target against brain injuries after SAH.
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Authors | Duo Chen, Xiang-tai Wei, Jun-hong Guan, Jiang-wei Yuan, Yu-tao Peng, Lei Song, Yun-hui Liu |
Journal | Acta neurochirurgica
(Acta Neurochir (Wien))
Vol. 154
Issue 8
Pg. 1469-76; discussion 1476
(Aug 2012)
ISSN: 0942-0940 [Electronic] Austria |
PMID | 22661329
(Publication Type: Journal Article)
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Chemical References |
- Anthracenes
- Claudin-5
- Cldn5 protein, rat
- Tjp1 protein, rat
- Zonula Occludens-1 Protein
- pyrazolanthrone
- JNK Mitogen-Activated Protein Kinases
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Topics |
- Animals
- Anthracenes
(therapeutic use)
- Apoptosis
(drug effects)
- Blood-Brain Barrier
(drug effects, metabolism, pathology)
- Brain
(metabolism, pathology)
- Claudin-5
(metabolism)
- Disease Models, Animal
- JNK Mitogen-Activated Protein Kinases
(antagonists & inhibitors, metabolism)
- Male
- Microscopy, Electron, Transmission
- Rats
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
- Subarachnoid Hemorrhage
(complications, drug therapy, metabolism, pathology)
- Tight Junctions
(drug effects, pathology, ultrastructure)
- Zonula Occludens-1 Protein
(metabolism)
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