Nitrite anion stimulates ischemic arteriogenesis involving NO metabolism.

Abstract

Nitric oxide (NO) is a potential regulator of ischemic vascular remodeling, and as such therapies augmenting its bioavailability may be useful for the treatment of ischemic tissue diseases. Here we examine the effect of administering the NO prodrug sodium nitrite on arteriogenesis activity during established tissue ischemia. Chronic hindlimb ischemia was induced by permanent unilateral femoral artery and vein ligation. Five days postligation; animals were randomized to control PBS or sodium nitrite (165 μg/kg) therapy twice daily. In situ vascular remodeling was measured longitudinally using SPY angiography and Microfil vascular casting. Delayed sodium nitrite therapy rapidly increased ischemic limb arterial vessel diameter and branching in a NO-dependent manner. SPY imaging angiography over time showed that nitrite therapy enhanced ischemic gracillis collateral vessel formation from the profunda femoris to the saphenous artery. Immunofluorescent staining of smooth muscle cell actin also confirmed that sodium nitrite therapy increased arteriogenesis in a NO-dependent manner. The NO prodrug sodium nitrite significantly increases arteriogenesis and reperfusion of established severe chronic tissue ischemia.

• Authors: Shyamal C Bir, Christopher B Pattillo, Sibile Pardue, Gopi K Kolluru, John Docherty, Dave Goyette, Peter Dvorsky, Christopher G Kevil
• Journal: American journal of physiology. Heart and circulatory physiology (Am J Physiol Heart Circ Physiol) Vol. 303 Issue 2 Pg. H178-88 (Jul 15 2012) ISSN: 1522-1539 [Electronic] United States
• PMID: 22610173 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Nitric Oxide Donors
• Prodrugs
• Nitric Oxide
• Sodium Nitrite

Topics

• Angiography
• Animals
• Chronic Disease
• Collateral Circulation (drug effects, physiology)
• Femoral Artery (drug effects, physiopathology, surgery)
• Femoral Vein (drug effects, physiopathology, surgery)
• Hindlimb (blood supply, drug effects, physiopathology)
• Ischemia (drug therapy, physiopathology)
• Mice
• Mice, Inbred C57BL
• Neovascularization, Physiologic (drug effects)
• Nitric Oxide (metabolism)
• Nitric Oxide Donors (administration & dosage)
• Prodrugs (administration & dosage)
• Regional Blood Flow (drug effects, physiology)
• Severity of Illness Index
• Sodium Nitrite (administration & dosage)