Abstract | BACKGROUND: Endothelial progenitor cells (EPCs) participate in vascular repair and angiogenesis. Thus, EPC transplantation into ischemic myocardium might improve cardiac function; however, the vast majority of cells die within a short period. The present study was designed to investigate whether exogenous erythropoietin (EPO) delivery could improve the survival of transplanted EPCs and enhance the efficiency of EPC-based cell therapy. METHODS: RESULTS: More enhanced green fluorescent protein-EPCs were found in the hearts treated with EPC + EPO than in those treated with EPC alone. The circulating EPC level was markedly elevated after EPC + EPO treatment compared with EPC application alone. Stromal cell-derived factor-1α and vascular endothelial growth factor were increased accordingly, along with increased microvessel density, decreased apoptosis, and reduced fibrosis in the peri- infarct myocardium. Left ventricular fractional shortening was greater and the interventricular septum was thicker after EPC + EPO treatment compared with EPC treatment alone. CONCLUSIONS:
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Authors | Yan Cheng, Ruoyu Hu, Lei Lv, Lin Ling, Shisen Jiang |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 176
Issue 1
Pg. e47-55
(Jul 2012)
ISSN: 1095-8673 [Electronic] United States |
PMID | 22595018
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Chemokine CXCL12
- Vascular Endothelial Growth Factor A
- Erythropoietin
- Green Fluorescent Proteins
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Topics |
- Animals
- Cell Movement
(drug effects)
- Cell Survival
(drug effects)
- Cell- and Tissue-Based Therapy
- Chemokine CXCL12
(metabolism)
- Electrocardiography
- Endothelium, Vascular
(cytology, drug effects, metabolism)
- Erythropoietin
(pharmacology)
- Green Fluorescent Proteins
(genetics)
- Male
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
(cytology, drug effects, metabolism)
- Mice
- Mice, Inbred BALB C
- Mice, Transgenic
- Models, Animal
- Myocardial Infarction
(metabolism, pathology, therapy)
- Neovascularization, Physiologic
(drug effects)
- Treatment Outcome
- Vascular Endothelial Growth Factor A
(metabolism)
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