Abstract |
DNA topoisomerase II alpha ( Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas.
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Authors | Yang Hong, Meng Sang, Chao Shang, Yi-xue Xue, Yun-hui Liu |
Journal | Neuroscience letters
(Neurosci Lett)
Vol. 518
Issue 2
Pg. 138-43
(Jun 19 2012)
ISSN: 1872-7972 [Electronic] Ireland |
PMID | 22569122
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antigens, Neoplasm
- Biomarkers, Tumor
- DNA-Binding Proteins
- RNA, Small Interfering
- DNA Topoisomerases, Type II
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Topics |
- Antigens, Neoplasm
(analysis, biosynthesis, genetics)
- Apoptosis
- Biomarkers, Tumor
(analysis, biosynthesis, genetics)
- Blotting, Western
- Brain Neoplasms
(enzymology, genetics, pathology)
- Cell Line, Tumor
- Cell Proliferation
- DNA Topoisomerases, Type II
(analysis, biosynthesis, genetics)
- DNA-Binding Proteins
(analysis, biosynthesis, genetics)
- Flow Cytometry
- Glioblastoma
(enzymology, genetics, pathology)
- Humans
- Neoplastic Stem Cells
(enzymology, pathology)
- RNA, Small Interfering
- Real-Time Polymerase Chain Reaction
- Transfection
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