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Quantitative analysis of topoisomerase II alpha and evaluation of its effects on cell proliferation and apoptosis in glioblastoma cancer stem cells.

Abstract
DNA topoisomerase II alpha (Topo-IIα) is an essential nuclear enzyme with its up-regulation demonstrated in different tumors. This study is to evaluate the expression of Topo-IIα in glioblastoma cancer stem cells (CSCs) and its effects on cell proliferation and apoptosis. We observed the expression differences of Topo-IIα gene transcript in CSCs and non-CSCs in glioblastoma cell line U87 by quantitative real-time PCR and western blot; we silenced Topo-IIα expression in U87 CSCs using Topo-IIα-specific siRNA to investigate the role of Topo-IIα in cell proliferation, cell cycle, and apoptosis. We found higher expression of Topo-IIα in CSCs than in non-CSCs. After using Topo-IIα-specific siRNA to silence the expression of Topo-IIα gene, we observed decreasing cell proliferation, cell cycle arrest, and induction of apoptosis in CSCs, which suggest that Topo-IIα contributes to both cell proliferation and/or cell survival. Topo-IIα may be a novel marker of cell proliferation and/or survival factor in glioblastoma CSCs. It may be a potential target that will open up new opportunities to develop therapeutic strategies for gliomas.
AuthorsYang Hong, Meng Sang, Chao Shang, Yi-xue Xue, Yun-hui Liu
JournalNeuroscience letters (Neurosci Lett) Vol. 518 Issue 2 Pg. 138-43 (Jun 19 2012) ISSN: 1872-7972 [Electronic] Ireland
PMID22569122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Chemical References
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • DNA-Binding Proteins
  • RNA, Small Interfering
  • DNA Topoisomerases, Type II
Topics
  • Antigens, Neoplasm (analysis, biosynthesis, genetics)
  • Apoptosis
  • Biomarkers, Tumor (analysis, biosynthesis, genetics)
  • Blotting, Western
  • Brain Neoplasms (enzymology, genetics, pathology)
  • Cell Line, Tumor
  • Cell Proliferation
  • DNA Topoisomerases, Type II (analysis, biosynthesis, genetics)
  • DNA-Binding Proteins (analysis, biosynthesis, genetics)
  • Flow Cytometry
  • Glioblastoma (enzymology, genetics, pathology)
  • Humans
  • Neoplastic Stem Cells (enzymology, pathology)
  • RNA, Small Interfering
  • Real-Time Polymerase Chain Reaction
  • Transfection

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