[Effect of Budesonide on Smad4, PDGF-A and PAI-1 in a rat model of pulmonary fibrosis].

Abstract

AIM:

To observe the effect of Budesonide (BUD) on TGF-β1, PDGF-A, Smad4 and PAI-1 in lung tissue in pulmonary fibrosis rats.

METHODS:

Forty-five adult female Wistar rats were randomly divided into normal solution (NS) group, BUD group and bleomycin (BLM) group. 9 g/L NaCl solution was instilled into the tratracheaes in NS group, and BLM were used in BUD group and BLM group. NS group and BLM group were inhaled with 9 g/L NaCl solution once everyday at day 0-6, and BUD group were used with BUD. Five rats in every group were killed at 7th, 14th 28th day. The appearances of alveolitis and fibrosis were displayed in HE and Masson staining. The expressions of TGF-β1, PDGF-A, Smad4 and PAI-1 in lung tissue were detected by immunohistochemistry.

RESULTS:

The extent of the alveolitis in BUD group at 7th, 14th day were significantly lower than that in BLM group(P<0.05). The fibrosis and the expressions of TGF-β1, PDGF-A, Smad4 and PAI-1 in lung tissues at 7th, 14th, 28th day, BUD group were significantly lower than BLM group(P<0.05).

CONCLUSION:

After inhaled BUD, the expressions of TGF-β1, PDGF-A, Smad4 and PAI-1 in lung tissue could be decreased, and the extent of alveolitis and pulmonary fibrosis could be improved in bleomycin-induced pulmonary fibrosis rats.

• Authors: Ju-ping Chen, Xian-ming Fan, Xiao-qin Zhan, Wen-jun Wang
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 28 Issue 5 Pg. 478-80 (May 2012) ISSN: 1007-8738 [Print] China
• PMID: 22558986 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Plasminogen Activator Inhibitor 1
• Platelet-Derived Growth Factor
• Smad4 Protein
• Smad4 protein, rat
• Transforming Growth Factor beta1
• platelet-derived growth factor A
• Budesonide

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Budesonide (pharmacology)
• Disease Models, Animal
• Female
• Plasminogen Activator Inhibitor 1 (analysis)
• Platelet-Derived Growth Factor (analysis)
• Pulmonary Fibrosis (drug therapy, metabolism)
• Rats
• Rats, Wistar
• Smad4 Protein (analysis)
• Transforming Growth Factor beta1 (analysis)