HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Cryptosporidium species in immunodeficient and immunocompetent patients of Valparaíso: a descriptive study].

AbstractOBJECTIVE:
Genetical characterization of human Cryptosporidium isolates to determine species diversity.
PATIENTS AND METHODS:
A cross-sectional study in Valparaiso, Chile, was performed. A total of 458 patients participated in the study: 259 immunodeficient (HIV, cancer, renal transplant hyper-IgM syndrome, HIV and unintended pregnancy) and 178 immunocompetent individuals provided stool samples and 21 patients bile samples.
RESULTS:
We obtained 29 (6.3%) positive samples. 25 (9.7%) derived from immunodeficient patients: 18 (7.3%) from HIV patients and 7 from patients with other immunodeficiencies. The remaining 4 (2.2%) samples originated from immunocompetent individuals. Cryptosporidium genotyping was performed by nested polymerase chain reaction (PCR) and restriction fragments length polymorphism and/or PCR followed by sequencing of the SSU rRNA from oocysts in stool samples. 4 species were identified: C. parvum, C. hominis, C. muris, and C. meleagridis. In immunodeficient patients, 16 C. parvum, 8 C. hominis, and 1 C. muris strain were identified. In immunocompetent participants, 3 C. hominis and 1 C. meleagridis isolate were found.
CONCLUSION:
The results indicate that zoonotic and anthroponotic transmission occurs and that C. parvum is the predominant species in our study population. Cryptosporidium species of zoonotic transmission accounted for 62% of the human infections detected in this study.
AuthorsPatricia Neira O, Nelson Muñoz S, Gonzalo Wilson L, M Elizabeth Barthel M, María J Rosales L, Carlos Henríquez R
JournalRevista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia (Rev Chilena Infectol) Vol. 29 Issue 1 Pg. 63-71 (Feb 2012) ISSN: 0716-1018 [Print] Chile
Vernacular TitleEspecies de Cryptosporidum en pacientes inmunodeficientes e inmunocompetentes de Valparaíso: Estudio descriptivo.
PMID22552514 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • RNA, Ribosomal, 18S
Topics
  • Adolescent
  • Adult
  • Aged
  • Child
  • Chile
  • Cross-Sectional Studies
  • Cryptosporidiosis (parasitology)
  • Cryptosporidium (classification, genetics, isolation & purification)
  • Female
  • Genotype
  • Humans
  • Immunocompetence
  • Immunocompromised Host
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Pregnancy
  • RNA, Ribosomal, 18S (genetics)
  • Young Adult

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: