Abstract | OBJECTIVE: Genetical characterization of human Cryptosporidium isolates to determine species diversity. PATIENTS AND METHODS: A cross-sectional study in Valparaiso, Chile, was performed. A total of 458 patients participated in the study: 259 immunodeficient (HIV, cancer, renal transplant hyper-IgM syndrome, HIV and unintended pregnancy) and 178 immunocompetent individuals provided stool samples and 21 patients bile samples. RESULTS: We obtained 29 (6.3%) positive samples. 25 (9.7%) derived from immunodeficient patients: 18 (7.3%) from HIV patients and 7 from patients with other immunodeficiencies. The remaining 4 (2.2%) samples originated from immunocompetent individuals. Cryptosporidium genotyping was performed by nested polymerase chain reaction (PCR) and restriction fragments length polymorphism and/or PCR followed by sequencing of the SSU rRNA from oocysts in stool samples. 4 species were identified: C. parvum, C. hominis, C. muris, and C. meleagridis. In immunodeficient patients, 16 C. parvum, 8 C. hominis, and 1 C. muris strain were identified. In immunocompetent participants, 3 C. hominis and 1 C. meleagridis isolate were found. CONCLUSION: The results indicate that zoonotic and anthroponotic transmission occurs and that C. parvum is the predominant species in our study population. Cryptosporidium species of zoonotic transmission accounted for 62% of the human infections detected in this study.
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Authors | Patricia Neira O, Nelson Muñoz S, Gonzalo Wilson L, M Elizabeth Barthel M, María J Rosales L, Carlos Henríquez R |
Journal | Revista chilena de infectologia : organo oficial de la Sociedad Chilena de Infectologia
(Rev Chilena Infectol)
Vol. 29
Issue 1
Pg. 63-71
(Feb 2012)
ISSN: 0716-1018 [Print] Chile |
Vernacular Title | Especies de Cryptosporidum en pacientes inmunodeficientes e inmunocompetentes de Valparaíso: Estudio descriptivo. |
PMID | 22552514
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adolescent
- Adult
- Aged
- Child
- Chile
- Cross-Sectional Studies
- Cryptosporidiosis
(parasitology)
- Cryptosporidium
(classification, genetics, isolation & purification)
- Female
- Genotype
- Humans
- Immunocompetence
- Immunocompromised Host
- Male
- Middle Aged
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Pregnancy
- RNA, Ribosomal, 18S
(genetics)
- Young Adult
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