Abstract | PURPOSE: To investigate the clinical and microbiological effectivity of intravitreal tigecycline in an experimental rabbit endophthalmitis model caused by imipenem resistant Acinetobacter baumannii. MATERIALS AND METHODS: Forty-eight eyes of 24 New Zealand white albino rabbits were divided into six groups (n=8 in each). The right eyes were divided into three groups and defined as infected group; left eyes were divided into three groups and defined as uninfected group. Infected group received 0.1 ml intravitreal A. baumannii suspension. Twenty-four hours after bacterial inoculation, group 1 received 1 mg/0.1 ml tigecycline and group 2 received 0.5 mg/0.1 ml tigecycline. Group 3 eyes received no treatment. In group 4, 0.1 ml of saline solution was injected. Groups 5 and 6 were received intravitreal tigecycline injection of 1 mg/0.1 ml and 0.5 mg/0.1 ml respectively. The eyes were enucleated for histopathological evaluation on the sixth day. Clinical and histological scoring systems were used to evaluate clinical and histological severity of the intraocular infection. RESULTS: The mean clinical scores of the six groups at the sixth day were 11±1.92, 12.4±6.2, 8.5±2.7, 0, 3±1.3, and 3±1.4 respectively. Mean histopathological scores were 7.8±2.8, 7.0±1.5, 5.6±1.4, 0, 0, and 0 respectively. There was no significant difference in mean clinical and histopathological scores of infected group (groups 1, 2 and 3). There was significant difference in mean clinical scores of groups 5 and 6 compared with group 4. Groups 4, 5 and 6 showed normal histological structure in histopathological evaluation and showed no significant difference. Microbiological cure was achieved in all infected eyes. CONCLUSIONS:
|
Authors | Fatih Horozoglu, Gokhan Metan, Ozkan Sever, Duygu Percin, Cumhur S Topal, Tansu Gonen, Kadircan H Keskinbora, Birol Topcu, Ates Yanyali |
Journal | Journal of chemotherapy (Florence, Italy)
(J Chemother)
Vol. 24
Issue 2
Pg. 101-6
(Apr 2012)
ISSN: 1973-9478 [Electronic] England |
PMID | 22546766
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Anti-Bacterial Agents
- Tigecycline
- Minocycline
|
Topics |
- Acinetobacter Infections
(drug therapy, microbiology)
- Acinetobacter baumannii
(drug effects, isolation & purification)
- Animals
- Anti-Bacterial Agents
(administration & dosage, pharmacology)
- Disease Models, Animal
- Endophthalmitis
(drug therapy, microbiology, pathology)
- Eye Infections, Bacterial
(drug therapy, microbiology, pathology)
- Female
- Humans
- Intravitreal Injections
- Male
- Minocycline
(administration & dosage, analogs & derivatives, pharmacology)
- Rabbits
- Tigecycline
- Treatment Outcome
|