Effect of triptolide on T-cell receptor beta variable gene mRNA expression in rats with collagen-induced arthritis.

Triptolide (TP) has been used in the treatment of rheumatoid arthritis (RA), but its mechanism of action is not understood. T-cell activation and associated release of cytokines appear to be major factors in the pathogenesis of RA. The overexpression of T-cell receptor (TCR) variable gene (V gene) fragments can cause the activation and infiltration of autoreactive T cells. This study examines the effects of TP on rats with collagen-induced arthritis (CIA). The levels of interleukin-10 (IL-10) in the serum were examined with ELISA. Compared to the CIA group, the levels of IL-10 were greater in the TP treatment group. Real-time quantitative polymerase chain reaction confirmed that the expression of TCR V beta (BV) 15 and TCR BV19 was increased in the CIA group, whereas in the TP treatment group, the expression was decreased. In this study, TP was found to enhance IL-10 levels and decrease the expression levels of TCR BV15 and TCR BV19. These changes might help explain the effectiveness of TP in the treatment of RA.
AuthorsJixi Wang, Aibing Wang, Heqing Zeng, Lantao Liu, Wenhao Jiang, Yongjie Zhu, Yudong Xu
JournalAnatomical record (Hoboken, N.J. : 2007) (Anat Rec (Hoboken)) Vol. 295 Issue 6 Pg. 922-7 (Jun 2012) ISSN: 1932-8494 [Electronic] United States
PMID22539421 (Publication Type: Journal Article)
CopyrightCopyright © 2012 Wiley Periodicals, Inc.
Chemical References
  • Anti-Inflammatory Agents, Non-Steroidal
  • Diterpenes
  • Epoxy Compounds
  • Phenanthrenes
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta
  • Interleukin-10
  • triptolide
  • Animals
  • Ankle Joint (metabolism, pathology, radiography)
  • Anti-Inflammatory Agents, Non-Steroidal (pharmacology)
  • Arthritis, Experimental (drug therapy, genetics, metabolism)
  • Disease Progression
  • Diterpenes (pharmacology)
  • Epoxy Compounds (pharmacology)
  • Female
  • Gene Expression (drug effects)
  • Interleukin-10 (blood)
  • Leukocytes, Mononuclear (chemistry, drug effects, pathology)
  • Organ Size (drug effects)
  • Peyer's Patches (drug effects, pathology)
  • Phenanthrenes (pharmacology)
  • RNA, Messenger (metabolism)
  • Rats
  • Rats, Wistar
  • Receptors, Antigen, T-Cell, alpha-beta (genetics)
  • Spleen (drug effects, pathology)
  • Thymus Gland (drug effects, pathology)

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