Blood graft composition after plerixafor injection in patients with NHL.

Abstract

BACKGROUND:

Plerixafor is used to mobilize CD34(+) hematopoietic stem cells from bone marrow to circulation. Limited data are available in regard to graft cellular content collected after plerixafor.

OBJECTIVES:

The aim of this study was to assess effects of plerixafor added to chemomobilization on graft CD34(+) cell subclasses, lymphocyte subsets, engraftment, and post-transplant course in non-Hodgkin lymphoma (NHL) patients.

METHODS:

Thirty-four patients with NHL were included. All patients received chemotherapy plus G-CSF to mobilize stem cells. Nineteen patients received plerixafor pre-emptively owing to poor mobilization or poor collection yields. The rest of the patients constituted the control group. Flow cytometric analyzes were performed from cryopreserved graft samples. Also, data on post-transplant engraftment and outcome were collected.

RESULTS:

The proportion of primitive stem cells (CD34(+)  CD133(+)  CD38(-) ) was significantly higher after the plerixafor injection when compared to the first collection in the control group. The amount of T cells (CD3(+) ), helper (CD3(+)  CD4(+) ) T subsets, and suppressor (CD3(+)  CD8(+) ) T subsets in the graft was all significantly higher in the plerixafor group. Also, the amount of NK cells (CD3(-)  CD16/56(+) ) was higher. Engraftment after high-dose therapy was comparable between the groups, but leukocyte and platelet count at 6 months were higher in patients receiving plerixafor-mobilized grafts.

CONCLUSION:

Plerixafor, when used pre-emptively in addition to chemomobilization, seems to mobilize more primitive CD34(+) stem cells, T lymphocytes, and NK cells. Whether these differences are associated with immune reconstitution, long-term engraftment, or patient outcomes needs to be evaluated in larger patient groups with longer follow-up.

• Authors: Ville Varmavuo, Pentti Mäntymaa, Tapio Nousiainen, Piia Valonen, Taru Kuittinen, Esa Jantunen
• Journal: European journal of haematology (Eur J Haematol) Vol. 89 Issue 2 Pg. 128-35 (Aug 2012) ISSN: 1600-0609 [Electronic] England
• PMID: 22536949 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 John Wiley & Sons A/S.

Chemical References

• Anti-HIV Agents
• Antigens, CD34
• Benzylamines
• Cyclams
• Heterocyclic Compounds
• Granulocyte Colony-Stimulating Factor
• plerixafor

Topics

• Adult
• Aged
• Anti-HIV Agents (administration & dosage)
• Antigens, CD34
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage)
• Benzylamines
• Cyclams
• Female
• Flow Cytometry
• Graft Survival (drug effects)
• Granulocyte Colony-Stimulating Factor (administration & dosage)
• Hematopoietic Stem Cell Mobilization (methods)
• Hematopoietic Stem Cells
• Heterocyclic Compounds (administration & dosage)
• Humans
• Killer Cells, Natural (pathology)
• Leukocyte Count
• Lymphoma, Non-Hodgkin (blood, pathology, therapy)
• Male
• Middle Aged
• Peripheral Blood Stem Cell Transplantation
• T-Lymphocytes (pathology)
• Transplantation, Autologous