Analysis of polymorphisms in T(H)2-associated genes in Russian patients with atopic bronchial asthma.

Abstract

BACKGROUND:

Bronchial asthma is a chronic respiratory disorder characterized by airway inflammation, airway hyperresponsiveness, and periodic reversible airway obstruction. Subtype 2 helperT cell (T(H)2) cytokines play an important role in the development of allergic airway inflammation in patients with bronchial asthma.

OBJECTIVE:

To investigate whether the single-nucleotide polymorphisms (SNPs) Ile75Val and Gln576Arg in the IL4RA gene, -33C>T in the IL4 gene, and Gly237Glu in the FCER1B gene contribute to the development and severity of atopic bronchial asthma in Russian patients from Moscow.

METHODS:

We analyzed DNA samples from 224 patients with atopic bronchial asthma and 172 healthy individuals. Genotyping was performed by primer extension followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

RESULTS:

We observed a moderate association between the Arg/Arg genotype of Gln576Arg and protection against asthma (odds ratio [OR], 0.16; P < .012) and a strong association between the T allele and TT genotype of -33C> and atopic bronchial asthma (OR, 1.91 and 4.65, respectively; P < .0001). Carriers of the C allele had a reduced risk of asthma (OR, 0.53; P < .0001). Furthermore, we found that the TT genotype of -33C>T correlated with higher concentrations of total serum immunoglobulin E and interleukin 4 than the CC and CT genotypes.

CONCLUSION:

We found an association between atopic bronchial asthma and the SNPs Gln576Arg in IL4RA and -33C>T in IL4. IL4RA and IL4 seem to be involved in the pathogenesis of asthma.

• Authors: E V Dmitrieva-Zdorova, O E Voronko, E A Latysheva, G I Storozhakov, A I Archakov
• Journal: Journal of investigational allergology & clinical immunology (J Investig Allergol Clin Immunol) Vol. 22 Issue 2 Pg. 126-32 ( 2012) ISSN: 1018-9068 [Print] Spain
• PMID: 22533235 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MS4A2 protein, human
• Receptors, IgE
• Interleukin-4
• Immunoglobulin E

Topics

• Adult
• Airway Obstruction (genetics, immunology, metabolism)
• Alleles
• Asthma (genetics, immunology, metabolism)
• Case-Control Studies
• Female
• Genetic Predisposition to Disease
• Genotype
• Humans
• Immunoglobulin E (genetics)
• Inflammation (genetics, metabolism)
• Interleukin-4 (genetics, metabolism)
• Male
• Moscow
• Odds Ratio
• Polymorphism, Single Nucleotide
• Receptors, IgE (genetics)
• Th2 Cells (immunology, metabolism, physiology)
• White People (genetics)