Liver
ischemia-reperfusion injury is a major cause of primary graft non-function or initial function failure post-
transplantation. In this study, we examined the effects of
sodium nitrite supplementation on liver IRI in either
Lactated Ringer's (LR)
solution or University of Wisconsin (
UW) solution. The syngeneic recipients of liver grafts were also treated with or without
nitrite by intra-peritoneal injection. Liver AST and LDH release were significantly reduced in both
nitrite-supplemented LR and UW preservation solutions compared to their controls. The protective effect of
nitrite was more efficacious with longer cold preservation times. Liver histological examination demonstrated better preserved morphology and architecture with
nitrite treatment. Hepatocellular apoptosis was significantly reduced in the
nitrite-treated livers compared their controls. Moreover, liver grafts with extended cold preservation time of 12 to 24 hours demonstrated improved liver tissue histology and function post-reperfusion with either the
nitrite-supplemented preservation
solution or in
nitrite-treated recipients. Interestingly, combined treatment of both the liver graft and recipient did not confer protection. Thus,
nitrite treatment affords significant protection from cold ischemic and
reperfusion injury to donor livers and improves liver graft acute function post-
transplantation. The results from this study further support the potential for
nitrite therapy to mitigate
ischemia-reperfusion injury in solid
organ transplantation.