Hexanic lipidosterolic extract of Serenoa repens inhibits the expression of two key inflammatory mediators, MCP-1/CCL2 and VCAM-1, in vitro.

Abstract

UNLABELLED:

What's known on the subject? and What does the study add? Pervasive inflammatory infiltrates, mainly composed of chronically activated T cells and monocytes/macrophages, have been observed in benign prostatic hyperplasia (BPH). Permixon®, a hexanic lipidosterolic extract of Serenoa repens (hexanic LSESr) used to treat urinary dysfunction in BPH patients, has anti-inflammatory activities. This paper provides new insights into the anti-inflammatory properties of Permixon®. We report that hexanic LSESr inhibits early steps of leukocyte infiltration in vitro by downregulating MCP-1/CCL2 and VCAM-1 expression.

OBJECTIVE:

To investigate the mechanisms by which hexanic lipidosterolic extract of Serenoa repens (hexanic LSESr) may prevent leukocyte infiltration in benign prostatic hyperplasia by studying its impact on monocyte chemoattractant protein 1/chemokine (C-C motif) ligand 2 (MCP-1/CCL2) and vascular cell adhesion molecule 1 (VCAM-1) expression in vitro.

MATERIALS AND METHODS:

After pretreatment with hexanic LSESr, human prostate (epithelial and myofibroblastic) cells and vascular endothelial cells were stimulated with proinflammatory cytokines. MCP-1/CCL2 and VCAM-1 mRNA expression was quantified by real-time PCR. ELISA kits were used to determine MCP-1/CCL2 levels in culture supernatants and VCAM-1 expression in living cells.

RESULTS:

Hexanic LSESr reduced MCP-1/CCL2 mRNA levels in both epithelial (BPH-1) and myofibroblastic (WPMY-1) prostate cell lines. Hexanic LSESr downregulated MCP1/CCL2 secretion by WPMY-1 cells in a concentration-dependent manner, more efficiently than Serenoa repens extracts obtained by supercritical carbon dioxide extraction. Hexanic LSESr inhibited tumour-necrosis-factor-α-induced MCP-1/CCL2 secretion by the human vascular endothelial cell line EAhy.926, as well as surface VCAM-1 protein expression, in a concentration-dependent manner.

CONCLUSIONS:

Hexanic LSESr impedes key steps of monocyte and T cell attraction and adherence by inhibiting MCP-1/CCL2 and VCAM-1 expression by human prostate and vascular cells in an inflammatory environment. These findings provide new insights into the anti-inflammatory effects of the hexanic lipidosterolic extract of Serenoa repens, Permixon®, in benign prostatic hyperplasia.

• Authors: Alain Latil, Christine Libon, Marie Templier, Didier Junquero, Frédérique Lantoine-Adam, Thien Nguyen
• Journal: BJU international (BJU Int) Vol. 110 Issue 6 Pt B Pg. E301-7 (Sep 2012) ISSN: 1464-410X [Electronic] England
• PMID: 22520557 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 BJU INTERNATIONAL.

Chemical References

• Chemokine CCL2
• Hexanes
• Plant Extracts
• Vascular Cell Adhesion Molecule-1

Topics

• Cells, Cultured
• Chemokine CCL2 (antagonists & inhibitors, biosynthesis)
• Hexanes (pharmacology)
• Humans
• Plant Extracts (pharmacology)
• Serenoa
• Vascular Cell Adhesion Molecule-1 (biosynthesis, drug effects)