Alkyl hydroperoxide reductase: a candidate Helicobacter pylori vaccine.

Abstract

Helicobacter pylori (H. pylori) is the most important etiological agent of chronic active gastritis, peptic ulcer disease and gastric cancer. The aim of this study was to evaluate the efficacy of alkyl hydroperoxide reductase (AhpC) and mannosylated AhpC (mAhpC) as candidate vaccines in the C57BL/6J mouse model of H. pylori infection. Recombinant AhpC was cloned, over-expressed and purified in an unmodified form and was also engineered to incorporate N and C-terminal mannose residues when expressed in the yeast Pichia pastoris. Mice were immunized systemically and mucosally with AhpC and systemically with mAhpC prior to challenge with H. pylori. Serum IgG responses to AhpC were determined and quantitative culture was used to determine the efficacy of vaccination strategies. Systemic prophylactic immunization with AhpC/alum and mAhpC/alum conferred protection against infection in 55% and 77.3% of mice, respectively. Mucosal immunization with AhpC/cholera toxin did not protect against infection and elicited low levels of serum IgG in comparison with systemic immunization. These data support the use of AhpC as a potential vaccine candidate against H. pylori infection.

• Authors: Avril A O'Riordan, Veronica Athie Morales, Linda Mulligan, Nazia Faheem, Henry J Windle, Dermot P Kelleher
• Journal: Vaccine (Vaccine) Vol. 30 Issue 26 Pg. 3876-84 (Jun 06 2012) ISSN: 1873-2518 [Electronic] Netherlands
• PMID: 22512976 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Adjuvants, Immunologic
• Alum Compounds
• Antibodies, Bacterial
• Bacterial Vaccines
• Glycoproteins
• Immunoglobulin G
• Recombinant Proteins
• Vaccines, Subunit
• Vaccines, Synthetic
• aluminum sulfate
• Peroxiredoxins

Topics

• Adjuvants, Immunologic (administration & dosage)
• Alum Compounds (administration & dosage)
• Animals
• Antibodies, Bacterial (blood)
• Bacterial Vaccines (administration & dosage, immunology)
• Disease Models, Animal
• Female
• Gene Expression
• Glycoproteins (immunology)
• Helicobacter Infections (immunology, prevention & control)
• Helicobacter pylori (enzymology, immunology)
• Humans
• Immunoglobulin G (blood)
• Mice
• Mice, Inbred C57BL
• Peroxiredoxins (immunology)
• Pichia (genetics)
• Recombinant Proteins (isolation & purification, metabolism)
• Vaccines, Subunit (administration & dosage, immunology)
• Vaccines, Synthetic (administration & dosage, immunology)