Vaccination with CpG-adjuvanted avian influenza virosomes promotes antiviral immune responses and reduces virus shedding in chickens.

Abstract

The use of virosomes as a vaccine platform has proven successful against several viruses. Here we examined the protective efficacy of a virosome-based vaccine consisting of avian influenza virus (AIV) A/Duck/Czech/56/H4N6 in chickens against a homologous AIV challenge. Virosomes adjuvanted with CpG-ODN or recombinant chicken interferon (IFN)-γ significantly reduced virus shedding after virus challenge. Furthermore, immunization with virosomes adjuvanted with CpG-ODN increased hemagglutination inhibition (HI) and virus-specific neutralizing serum antibodies, as well as virus-specific serum IgG and mucosal IgA responses. We also found a significant increase in the expression of type I and II interferon genes in the protected birds following virus challenge. In summary, this study demonstrated the ability of virosomes adjuvanted with CpG-ODN to reduce AIV shedding, and elicit virus-specific protective antibody responses in vaccinated birds.

• Authors: Amirul I Mallick, Raveendra R Kulkarni, Michael St Paul, Payvand Parvizi, Eva Nagy, Shahriar Behboudi, Shayan Sharif
• Journal: Viral immunology (Viral Immunol) Vol. 25 Issue 3 Pg. 226-31 (Jun 2012) ISSN: 1557-8976 [Electronic] United States
• PMID: 22512311 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Neutralizing
• Antibodies, Viral
• CPG-oligonucleotide
• Influenza Vaccines
• Oligodeoxyribonucleotides
• Vaccines, Virosome

Topics

• Adjuvants, Immunologic (administration & dosage)
• Animals
• Antibodies, Neutralizing (blood)
• Antibodies, Viral (blood)
• Chickens (immunology)
• Influenza A virus (immunology)
• Influenza Vaccines (administration & dosage)
• Influenza in Birds (immunology, prevention & control, virology)
• Oligodeoxyribonucleotides (administration & dosage, immunology)
• Poultry Diseases (immunology, prevention & control)
• Vaccination
• Vaccines, Virosome (administration & dosage, immunology)
• Virus Shedding (immunology)