GSTP1 gene methylation profiles in Helicobacter pylori (+) and (-) antral intestinal metaplasia and distal gastric tumour patients in Turkish population.

Abstract

BACKGROUND/AIMS:

Gastric cancer (GC) is the second most common malignancy worldwide, with a high mortality rate. The incidence of GC has declined in the western countries during the last decades. The glutathione S-transferases comprise a group of enzymes that are critical in the detoxification of carcinogens. In this study we aimed at the relationship GSTP-1 methylation in patients with intestinal metaplasia with and without Helicobacter pylori infection, gastric cancer and controls.

METHODOLOGY:

The methylation status of GSTP1 gene was analyzed by methylation specific PCR after bisulfate modification in H. pylori (+) (n=25) and (-) (n=25) intestinal metaplasia (IM) patients, GC (n=25) and control subjects (n=15) between September 2009 to November 2011.

RESULTS:

During the study period 90 patients who underwent endoscopic examination were included in the study. When we considered the GSTP1 gene methylation profile in all of the groups; 26 (28%)patients had methylated GSTP1 gene, 31 (34%) patients had unmethylated GSTP1 gene and 33 (36%) patients had heterogeneously methylated GSTPI gene.

CONCLUSIONS:

GSTP1 gene methylation profile is not appropriate for early diagnosis of cases with gastric cancer.

• Authors: Gulcan Asik-Sen, Elmas Kasap, Seda Orenay-Boyacioglu, Mehmet Korkmaz, Erkan Kahraman, Belkis Unsal, Elif Yuksel-Saritas, Hakan Yuceyar
• Journal: Hepato-gastroenterology (Hepatogastroenterology) 2012 Nov-Dec Vol. 59 Issue 120 Pg. 2664-7 ISSN: 0172-6390 [Print] Greece
• PMID: 22497954 (Publication Type: Journal Article)

Chemical References

• GSTP1 protein, human
• Glutathione S-Transferase pi

Topics

• Adult
• Biopsy
• Case-Control Studies
• Chi-Square Distribution
• DNA Methylation
• Early Detection of Cancer
• Female
• Gastroscopy
• Genetic Predisposition to Disease
• Glutathione S-Transferase pi (genetics)
• Helicobacter Infections (enzymology, genetics, microbiology, pathology)
• Helicobacter pylori (isolation & purification)
• Humans
• Male
• Metaplasia
• Middle Aged
• Phenotype
• Polymerase Chain Reaction
• Predictive Value of Tests
• Prognosis
• Pyloric Antrum (enzymology, microbiology, pathology)
• Stomach Neoplasms (enzymology, genetics, microbiology, pathology)
• Turkey