Salmonella enterica induces joint inflammation and expression of interleukin-17 in draining lymph nodes early after onset of enterocolitis in mice.

Abstract

In developing countries, one-third of reactive arthritis (ReA) cases are associated with Salmonella enterocolitis; nevertheless, there is no animal model for studying this pathology. Here we induced a self-limiting Salmonella enterica serovar Enteritidis enterocolitis in mice to analyze the onset of ReA. BALB/c mice received orally 20 μg of streptomycin 24 h before intragastric inoculation of a low dose (3 × 10(3) to 4 × 10(3) CFU) of S. Enteritidis. In response to Salmonella infection, a 30-fold increase in the expression of interleukin-17 (IL-17), measured by quantitative PCR, was observed in mesenteric lymph nodes 5 days postinfection. At this time synovitis was already evident, and concomitantly, a significant increase in joint tumor necrosis factor alpha (TNF-α) was detected by enzyme-linked immunosorbent assay (ELISA). The early development of joint lesions was accompanied by an increased expression of IL-17 in inguinal and popliteal lymph nodes. Infection with 10(7) CFU of an isogenic ΔinvG mutant bearing a defective type III secretion system of Salmonella encoded in the pathogenicity island 1 apparatus (TTSS-1) induced enterocolitis histologically similar to that triggered by the wild-type strain. Interestingly, despite the higher infective dose used, the mutant did not trigger intestinal IL-17. Moreover, no synovitis was observed in mice suffering ΔinvG enterocolitis. Neutralization of IL-17 in mice infected with S. Enteritidis prevented both synovitis and the increment of TNF-α in the joints, suggesting that IL-17 participates in the generation of Salmonella-induced ReA through the induction of TNF-α in the joints.

• Authors: Mariángeles Noto Llana, Sebastián Hernán Sarnacki, María Victoria Vázquez, Alejandra Sonia Gartner, Mónica Nancy Giacomodonato, María Cristina Cerquetti
• Journal: Infection and immunity (Infect Immun) Vol. 80 Issue 6 Pg. 2231-9 (Jun 2012) ISSN: 1098-5522 [Electronic] United States
• PMID: 22493084 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-17
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Arthritis, Infectious (etiology, immunology)
• Enterocolitis (immunology, microbiology, pathology)
• Female
• Gene Expression Profiling
• Gene Expression Regulation, Bacterial (physiology)
• Interleukin-17 (genetics, metabolism)
• Intestines (pathology)
• Lymph Nodes (immunology)
• Mice
• Mice, Inbred BALB C
• Salmonella Infections, Animal (immunology, pathology)
• Salmonella enterica (pathogenicity)
• Tumor Necrosis Factor-alpha (genetics, metabolism)