Abstract | BACKGROUND AND AIM:
Cysteamine is a reducing aminothiol used for inducing duodenal ulcer through mechanisms of oxidative stress related to thiol-derived H(2)O(2) reaction. Cochinchina momordica saponins have been suggested to be protective against various gastric diseases based on their cytoprotective and anti-inflammatory mechanisms. This study was aimed to document the preventive effects of Cochinchina momordica seed extract against cysteamine-induced duodenal ulcer as well as the elucidation of its pharmacological mechanisms. METHODS: Cochinchina momordica seed extract (50, 100, 200 mg/kg) was administrated intragastrically before cysteamine administration, after which the incidence of the duodenal ulcer, ulcer size, serum gastrin level, and the ratio of reduced glutathione (GSH)/ oxidized glutathione disulfide ( GSSG) as well as biochemical and molecular measurements of cytoplasmic phospholipase A(2) (cPLA(2)), cyclooxygenase-2 (COX-2), 5-lipoxygenase and the expression of proinflammatory genes including IL-1β, IL-6, COX-2 were measured in rat model. Additional experiments of electron spin resonance measurement and the changes of glutathione were performed. RESULTS: Cochinchina momordica seed extract effectively prevented cysteamine-induced duodenal ulcer in a dose-dependent manner as reflected with significant decreases in either duodenal ulcerogenesis or perforation accompanied with significantly decreased in serum gastrin in addition to inflammatory mediators including cPLA(2), COX-2, and 5-lipoxygenase. Cochinchina momordica seed extract induced the expression of γ- glutamylcysteine synthetase (γ-GCS)-related glutathione synthesis as well as significantly reduced the expression of cPLA(2). Cochinchina momordica seed extract preserved reduced glutathione through increased expressions of γ-GCS. CONCLUSION:
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Authors | Ki-Seok Choi, Eun-Hee Kim, Hua Hong, Chan Young Ock, Jeong Sang Lee, Joo-Hyun Kim, Ki-Baik Hahm |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 27 Suppl 3
Pg. 13-22
(Apr 2012)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 22486866
(Publication Type: Journal Article)
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Copyright | © 2012 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
Chemical References |
- Anti-Ulcer Agents
- Antioxidants
- Enzyme Activators
- Gastrins
- Inflammation Mediators
- Lipoxygenase Inhibitors
- Plant Extracts
- Cysteamine
- Arachidonate 5-Lipoxygenase
- Cyclooxygenase 2
- Ptgs2 protein, rat
- Phospholipases A2, Cytosolic
- Glutamate-Cysteine Ligase
- Glutathione
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Topics |
- Animals
- Anti-Ulcer Agents
(isolation & purification, pharmacology)
- Antioxidants
(pharmacology)
- Arachidonate 5-Lipoxygenase
(metabolism)
- Cell Line
- Cyclooxygenase 2
(metabolism)
- Cysteamine
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Duodenal Ulcer
(chemically induced, enzymology, pathology, prevention & control)
- Duodenum
(drug effects, enzymology, pathology)
- Enzyme Activation
- Enzyme Activators
(isolation & purification, pharmacology)
- Gastrins
(blood)
- Glutamate-Cysteine Ligase
(metabolism)
- Glutathione
(metabolism)
- Inflammation Mediators
(metabolism)
- Intestinal Mucosa
(drug effects, enzymology, pathology)
- Lipoxygenase Inhibitors
(isolation & purification, pharmacology)
- Male
- Momordica
(chemistry)
- Oxidation-Reduction
- Oxidative Stress
(drug effects)
- Phospholipases A2, Cytosolic
(antagonists & inhibitors, metabolism)
- Plant Extracts
(isolation & purification, pharmacology)
- Rats
- Rats, Sprague-Dawley
- Seeds
- Time Factors
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