HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Attenuation of cysteamine-induced duodenal ulcer with Cochinchina momordica seed extract through inhibiting cytoplasmic phospholipase A2/5-lipoxygenase and activating γ-glutamylcysteine synthetase.

AbstractBACKGROUND AND AIM:
Cysteamine is a reducing aminothiol used for inducing duodenal ulcer through mechanisms of oxidative stress related to thiol-derived H(2)O(2) reaction. Cochinchina momordica saponins have been suggested to be protective against various gastric diseases based on their cytoprotective and anti-inflammatory mechanisms. This study was aimed to document the preventive effects of Cochinchina momordica seed extract against cysteamine-induced duodenal ulcer as well as the elucidation of its pharmacological mechanisms.
METHODS:
Cochinchina momordica seed extract (50, 100, 200 mg/kg) was administrated intragastrically before cysteamine administration, after which the incidence of the duodenal ulcer, ulcer size, serum gastrin level, and the ratio of reduced glutathione (GSH)/oxidized glutathione disulfide (GSSG) as well as biochemical and molecular measurements of cytoplasmic phospholipase A(2) (cPLA(2)), cyclooxygenase-2 (COX-2), 5-lipoxygenase and the expression of proinflammatory genes including IL-1β, IL-6, COX-2 were measured in rat model. Additional experiments of electron spin resonance measurement and the changes of glutathione were performed.
RESULTS:
Cochinchina momordica seed extract effectively prevented cysteamine-induced duodenal ulcer in a dose-dependent manner as reflected with significant decreases in either duodenal ulcerogenesis or perforation accompanied with significantly decreased in serum gastrin in addition to inflammatory mediators including cPLA(2), COX-2, and 5-lipoxygenase. Cochinchina momordica seed extract induced the expression of γ-glutamylcysteine synthetase (γ-GCS)-related glutathione synthesis as well as significantly reduced the expression of cPLA(2). Cochinchina momordica seed extract preserved reduced glutathione through increased expressions of γ-GCS.
CONCLUSION:
Cochinchina momordica seed extracts exerted significantly protective effect against cysteamine-induced duodenal ulcer by either cPLA2 inhibition or glutathione preservation.
AuthorsKi-Seok Choi, Eun-Hee Kim, Hua Hong, Chan Young Ock, Jeong Sang Lee, Joo-Hyun Kim, Ki-Baik Hahm
JournalJournal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 27 Suppl 3 Pg. 13-22 (Apr 2012) ISSN: 1440-1746 [Electronic] Australia
PMID22486866 (Publication Type: Journal Article)
Copyright© 2012 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.
Chemical References
  • Anti-Ulcer Agents
  • Antioxidants
  • Enzyme Activators
  • Gastrins
  • Inflammation Mediators
  • Lipoxygenase Inhibitors
  • Plant Extracts
  • Cysteamine
  • Arachidonate 5-Lipoxygenase
  • Cyclooxygenase 2
  • Ptgs2 protein, rat
  • Phospholipases A2, Cytosolic
  • Glutamate-Cysteine Ligase
  • Glutathione
Topics
  • Animals
  • Anti-Ulcer Agents (isolation & purification, pharmacology)
  • Antioxidants (pharmacology)
  • Arachidonate 5-Lipoxygenase (metabolism)
  • Cell Line
  • Cyclooxygenase 2 (metabolism)
  • Cysteamine
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Duodenal Ulcer (chemically induced, enzymology, pathology, prevention & control)
  • Duodenum (drug effects, enzymology, pathology)
  • Enzyme Activation
  • Enzyme Activators (isolation & purification, pharmacology)
  • Gastrins (blood)
  • Glutamate-Cysteine Ligase (metabolism)
  • Glutathione (metabolism)
  • Inflammation Mediators (metabolism)
  • Intestinal Mucosa (drug effects, enzymology, pathology)
  • Lipoxygenase Inhibitors (isolation & purification, pharmacology)
  • Male
  • Momordica (chemistry)
  • Oxidation-Reduction
  • Oxidative Stress (drug effects)
  • Phospholipases A2, Cytosolic (antagonists & inhibitors, metabolism)
  • Plant Extracts (isolation & purification, pharmacology)
  • Rats
  • Rats, Sprague-Dawley
  • Seeds
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: