Effect of cultured autologous oral keratinocyte suspension in fibrin glue on oral wound healing in rabbits.

The effect of cultured autologous oral keratinocyte suspension in fibrin glue on the healing of surgically produced oral mucosal wounds was assessed in the rabbit model. Using the light microscope and a digital image analysis system, the epithelization parameters (marginal epithelization and percentage of wound re-epithelization) were measured in haematoxylin-eosin stained sections of the wound area and compared with those of wounds treated with fibrin glue alone and untreated ones. The epithelization was significantly higher in keratinocytes plus fibrin glue-treated wounds on postoperative days 3 and 7. No significant differences were observed on postoperative day 1, when the healing process had just begun, and on postoperative day 14, when re-epithelization was completed or nearly completed in all groups. The inflammatory infiltration of the wounded mucosa was weakest in keratinocyte-treated wounds and strongest in untreated wounds. In conclusion, suspension of cultured autologous oral keratinocytes in fibrin glue significantly accelerates oral wound healing in the rabbit model and could be beneficial in the treatment of oral wounds in patients.
AuthorsG J Lis, J Zarzecka, J A Litwin, E Jasek, T Cichocki, J Zapała
JournalInternational journal of oral and maxillofacial surgery (Int J Oral Maxillofac Surg) Vol. 41 Issue 9 Pg. 1146-52 (Sep 2012) ISSN: 1399-0020 [Electronic] Denmark
PMID22456103 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • Fibrin Tissue Adhesive
  • Animals
  • Biological Dressings
  • Cells, Cultured
  • Combined Modality Therapy
  • Fibrin Tissue Adhesive (administration & dosage)
  • Keratinocytes (cytology, transplantation)
  • Mouth Mucosa (drug effects, surgery)
  • Rabbits
  • Transplantation, Autologous
  • Wound Healing (drug effects, physiology)

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