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Antiproliferative and antiangiogenic properties of horse chestnut extract.

Abstract
This study was designed to examine the in vitro antiproliferative effect of the horse chestnut extract (HCE) on cancer cell lines. Furthermore, we have investigated the in vitro effect of HCE on some angiogenic events by using human umbilical vein endothelial cells. The cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and anchorage-independent growth by colony-forming assay. To understand the growth inhibitory effects, carcinoma cell lines (Jurkat, CEM, HeLa, and MCF-7) were treated with various concentrations of HCE. Incubation of Jurkat, CEM, HeLa, and MCF-7 cancer cells with HCE at 125 µg/mL for 72 h caused 93.7%, 32.3%, 20.4% and 40.4% reduction in cell survival. Colony-forming assay also confirmed growth-inhibitory effects of the compound studied. In HeLa HCE-treated cells, we found a significant increase in cells having sub-G(0) /G(1) DNA content which is considered to be a marker of apoptotic cell death. Apoptosis was also further confirmed by DNA fragmentation analysis.Furthermore, HCE inhibited migration of human umbilical vein endothelial cells as well as decreased secretion of matrix metalloproteinase and vascular endothelial growth factor.In conclusion, the present study has assessed the in vitro antiproliferative/antiangiogenic potential of HCE. These results generate a rationale for in vivo efficacy studies with horse chestnut in preclinical cancer models.
AuthorsGabriela Mojžišová, Ján Mojžiš, Martina Pilátová, Lenka Varinská, Lenka Ivanová, Ladislav Strojný, Ján Richnavský
JournalPhytotherapy research : PTR (Phytother Res) Vol. 27 Issue 2 Pg. 159-65 (Feb 2013) ISSN: 1099-1573 [Electronic] England
PMID22451355 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 John Wiley & Sons, Ltd.
Chemical References
  • Angiogenesis Inhibitors
  • Plant Extracts
  • Vascular Endothelial Growth Factor A
Topics
  • Aesculus (chemistry)
  • Angiogenesis Inhibitors (pharmacology)
  • Apoptosis (drug effects)
  • Cell Line, Tumor
  • Cell Movement (drug effects)
  • Cell Proliferation (drug effects)
  • DNA Fragmentation (drug effects)
  • Human Umbilical Vein Endothelial Cells (drug effects)
  • Humans
  • Plant Extracts (pharmacology)
  • Vascular Endothelial Growth Factor A (metabolism)

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