[Adipose tissue sample preparation for scanning electron microscopy].

Abstract

Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy (SEM). We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids, phospholipids protein, and hence the membrane structure. Also by extending the dehydration time to fully replace the organic solvents, we achieved satisfactory results for SEM of adipose samples.

• Authors: Fang Cui, Chao Liu, Jian-Min Yang, Li Wang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 32 Issue 3 Pg. 435-6 (Mar 2012) ISSN: 1673-4254 [Print] China
• PMID: 22446002 (Publication Type: English Abstract, Journal Article)

Topics

• Adipose Tissue (ultrastructure)
• Animals
• Female
• Humans
• Microscopy, Electron, Scanning
• Rats
• Rats, Sprague-Dawley
• Specimen Handling (methods)