Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors.

Abstract

Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.

• Authors: Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L Epstein, Juan Ramón Arbiza
• Journal: Journal of veterinary science (J Vet Sci) Vol. 13 Issue 1 Pg. 59-65 (Mar 2012) ISSN: 1976-555X [Electronic] Korea (South)
• PMID: 22437537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Viral Proteins
• Viral Vaccines
• bovine herpesvirus type-1 glycoproteins

Topics

• Animals
• Antibodies, Viral (blood)
• Blotting, Western (veterinary)
• Cattle
• Female
• Genetic Vectors (immunology)
• Herpesvirus 1, Bovine (genetics, immunology)
• Herpesvirus 1, Human (genetics, immunology)
• Immunity, Humoral (immunology)
• Immunization (methods, veterinary)
• Infectious Bovine Rhinotracheitis (immunology, prevention & control, virology)
• Mice
• Mice, Inbred BALB C
• Neutralization Tests (veterinary)
• Specific Pathogen-Free Organisms
• Viral Proteins (genetics, immunology)
• Viral Vaccines (immunology)