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Multiple ABC transporters are involved in the acquisition of petrobactin in Bacillus anthracis.

Abstract
In Bacillus anthracis the siderophore petrobactin is vital for iron acquisition and virulence. The petrobactin-binding receptor FpuA is required for these processes. Here additional components of petrobactin reacquisition are described. To identify these proteins, mutants of candidate permease and ATPase genes were generated allowing for characterization of multiple petrobactin ATP-binding cassette (ABC)-import systems. Either of two distinct permeases, FpuB or FatCD, is required for iron acquisition and play redundant roles in petrobactin transport. A mutant strain lacking both permeases, ΔfpuBΔfatCD, was incapable of using petrobactin as an iron source and exhibited attenuated virulence in a murine model of inhalational anthrax infection. ATPase mutants were generated in either of the permease mutant backgrounds to identify the ATPase(s) interacting with each individual permease channel. Mutants lacking the FpuB permease and FatE ATPase (ΔfpuBΔfatE) and a mutant lacking the distinct ATPases FpuC and FpuD generated in the ΔfatCD background (ΔfatCDΔfpuCΔfpuD) displayed phenotypic characteristics of a mutant deficient in petrobactin import. A mutant lacking all three of the identified ATPases (ΔfatEΔfpuCΔfpuD) exhibited the same growth defect in iron-depleted conditions. Taken together, these results provide the first description of the permease and ATPase proteins required for the import of petrobactin in B. anthracis.
AuthorsShandee D Dixon, Brian K Janes, Alexandra Bourgis, Paul E Carlson Jr, Philip C Hanna
JournalMolecular microbiology (Mol Microbiol) Vol. 84 Issue 2 Pg. 370-82 (Apr 2012) ISSN: 1365-2958 [Electronic] England
PMID22429808 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Copyright© 2012 Blackwell Publishing Ltd.
Chemical References
  • ATP-Binding Cassette Transporters
  • Benzamides
  • Virulence Factors
  • petrobactin
  • Iron
Topics
  • ATP-Binding Cassette Transporters (genetics, metabolism)
  • Animals
  • Anthrax (microbiology, pathology)
  • Bacillus anthracis (genetics, metabolism, pathogenicity)
  • Benzamides (metabolism)
  • Disease Models, Animal
  • Gene Deletion
  • Iron (metabolism)
  • Mice
  • Models, Biological
  • Mutagenesis
  • Survival Analysis
  • Virulence
  • Virulence Factors (genetics, metabolism)

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