Abstract | OBJECTIVE: DESIGN: Case-control study conducted from 2002 to 2005. SETTING: Policlinico Umberto I, "Sapienza" University of Rome and Italian National Institute for Health, Rome. PATIENT(S): Italian women (N = 343), with laparoscopic diagnosis and histologic confirmation of the presence (cases, N = 181) or the absence (controls, N = 162) of endometriosis. INTERVENTION(S): Genomic DNA extraction, multiplex polymerase chain reaction (PCR), and restriction fragment length polymorphism analysis. Determination of serum concentrations of selected PCBs by ion-trap mass spectrometry (subgroup, 63 cases and 63 controls). MAIN OUTCOME MEASURE(S):
Endometriosis diagnosis by laparoscopy, GST genotypes, serum PCB levels. RESULT(S): The genotype distributions of GSTM1, GSTA1, and GSTP1 did not show any statistically significant difference between cases and controls. The GSTT1 null genotype was negatively associated with the disease. The GSTP1 wild-type genotype in the presence of medium-high blood levels of PCB153, total PCBs, or of high levels of PCB180 significantly increased the risk of endometriosis, suggesting a multiplicative interaction. CONCLUSION(S): The GSTs polymorphisms per se do not increase the risk of developing endometriosis. However, a gene-environment interaction was observed for GSTP1(Ile/Ile) and GSTM1 null genotypes, modulating the effect of PCB153, PCB180, and of total PCBs on disease risk.
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Authors | Susanna Vichi, Emanuela Medda, Anna Maria Ingelido, Annamaria Ferro, Serena Resta, Maria Grazia Porpora, Annalisa Abballe, Lorenza Nisticò, Elena De Felip, Simonetta Gemma, Emanuela Testai |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 97
Issue 5
Pg. 1143-51.e1-3
(May 2012)
ISSN: 1556-5653 [Electronic] United States |
PMID | 22424617
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Environmental Pollutants
- Polychlorinated Biphenyls
- glutathione S-transferase T1
- GSTA1 protein, human
- GSTP1 protein, human
- Glutathione S-Transferase pi
- Glutathione Transferase
- glutathione S-transferase M1
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Topics |
- Adult
- Case-Control Studies
- Chi-Square Distribution
- Endometriosis
(chemically induced, enzymology, etiology, genetics, pathology)
- Environmental Pollutants
(adverse effects, blood)
- Female
- Gene Frequency
- Gene-Environment Interaction
- Genetic Predisposition to Disease
- Glutathione S-Transferase pi
(genetics)
- Glutathione Transferase
(genetics)
- Humans
- Italy
- Laparoscopy
- Logistic Models
- Mass Spectrometry
- Odds Ratio
- Polychlorinated Biphenyls
(adverse effects, blood)
- Polymerase Chain Reaction
- Risk Assessment
- Risk Factors
- Time Factors
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