Induction of immune tolerance in mice with a novel mucosal nanoemulsion adjuvant and self-antigen.

Abstract

AIM:

The aim of this study was to investigate the impact of a novel nanoemulsion (NE) adjuvant, a soybean oil emulsion, on autoimmune response. To this end, we used murine thyroglobulin (mTg)-induced experimental autoimmune thyroiditis in mice as a study model.

MATERIALS & METHODS:

Mice received NE or NE + mTg by nasal delivery. At 1 week after the second nasal delivery of NE with or without mTg, all mice were immunized with mTg and lipopolysaccharides to induce experimental autoimmune thyroiditis.

RESULTS:

Compared with controls, mTg-NE-treated mice had much more antigens accumulated in the nasal passage and thymus and developed a milder form of thyroiditis. This was accompanied by an increase in IL-10, IL-17 and reduced IFN-γ. The production of anti-mTg antibodies was significantly decreased in mTg-NE-treated mice. The percentage of Tregs in cervical lymph nodes was higher in mTg-NE-treated mice than NE-treated mice. Furthermore, Foxp3 and TGF-β levels were prominently enhanced in mTg-NE-treated mice.

CONCLUSION:

This study indicates that a low dose of mTg in NE can significantly enhance antigen uptake and Tregs, resulting in inhibition of experimental autoimmune thyroiditis development.

• Authors: Su He Wang, Yongyi Fan, Paul E Makidon, Zhengyi Cao, James R Baker
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 7 Issue 6 Pg. 867-76 (Jun 2012) ISSN: 1748-6963 [Electronic] England
• PMID: 22420425 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Adjuvants, Immunologic
• Autoantibodies
• Emulsions
• Forkhead Transcription Factors
• Foxp3 protein, mouse
• Interleukin-17
• RNA, Messenger
• Transforming Growth Factor beta
• anti-thyroglobulin
• Interleukin-10
• Soybean Oil
• Interferon-gamma
• Thyroglobulin

Topics

• Adjuvants, Immunologic (administration & dosage, therapeutic use)
• Administration, Intranasal
• Animals
• Autoantibodies (immunology)
• Emulsions (administration & dosage, therapeutic use)
• Female
• Forkhead Transcription Factors (genetics, immunology)
• Gene Expression
• Immune Tolerance
• Interferon-gamma (immunology)
• Interleukin-10 (immunology)
• Interleukin-17 (immunology)
• Mice
• Mice, Inbred CBA
• RNA, Messenger (genetics)
• Soybean Oil (administration & dosage, immunology, therapeutic use)
• T-Lymphocytes, Regulatory (immunology)
• Thyroglobulin (administration & dosage, immunology)
• Thyroid Gland (immunology, metabolism, pathology)
• Thyroiditis, Autoimmune (immunology, pathology, prevention & control)
• Transforming Growth Factor beta (genetics, immunology)