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Novel protein of IBP from silkworm, Bombyx mori, involved in cytoplasmic polyhedrosis virus infection.

Abstract
In the present study, the full-length cDNA of a novel insulin-related peptide-binding protein (named BmIBP2) was identified from silkworm, Bombyx mori, using rapid amplification of cDNA ends. The full-length cDNA of BmIBP2 is 1293 bp, consisting of a 5'-terminal untranslated region (UTR) of 61 bp, and a 3'-UTR of 335 bp with a poly-adenylation signal sequence AATAAA and a poly (A) tail. The BmIBP2 cDNA encodes a polypeptide of 298 amino acids, including an IG domain and an IGc2 domain, with a theoretical isoelectric point of 5.73 and a predicted molecular weight of 33.1 kDa. The BmIBP2 also has a signal peptide of 23 amino acids and a potential N-glycosylation site. The sequence similarity and phylogenic analysis indicated that BmIBP2 belongs to the group of invertebrates IBP and is closer to IGFBP7 than to the other IGFBPs in vertebrates. These findings suggest that BmIBP2 is a putative homolog of vertebrate endocrine factor IGFBP7 and has a functional similarity. By fluorescent quantitative real-time polymerase chain reaction, mRNA transcripts of BmIBP2 were mainly detected in the midgut but were hardly detectable in the hemocytes, vasa mucosa, fat body, silk gland, head, testicle, ovary, and spiracle. After the silkworm larvae were infected by B. mori cytoplasmic polyhedrosis virus (BmCPV), a significant up-regulation in the relative expression level of BmIBP2 was found. All the results suggested that BmIBP2 is a novel protein that plays an important role in the insulin-signal pathway and in the immune response of silkworm to BmCPV infection.
AuthorsKun Gao, Xiang-yuan Deng, He-Ying Qian, Ping Wu, Guang-xing Qin, Ting Liu, Zhong-yuan Shen, Xi-jie Guo
JournalJournal of invertebrate pathology (J Invertebr Pathol) Vol. 110 Issue 1 Pg. 83-91 (May 2012) ISSN: 1096-0805 [Electronic] United States
PMID22401767 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 Elsevier Inc. All rights reserved.
Chemical References
  • DNA, Complementary
  • Insect Proteins
  • Insulin-Like Growth Factor Binding Proteins
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bombyx (genetics, immunology, virology)
  • DNA, Complementary (genetics)
  • Gene Expression Profiling
  • Insect Proteins (genetics, immunology)
  • Insulin-Like Growth Factor Binding Proteins (genetics, immunology)
  • Molecular Sequence Data
  • Phylogeny
  • Real-Time Polymerase Chain Reaction
  • Reoviridae (immunology)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis

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