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New enzyme-linked immunosorbent assay for detection of antibodies against hepatitis delta virus using a hepatitis delta antigen derived from a Taiwanese clone and comparison to the Abbott radioimmunoassay.

Abstract
An anti-hepatitis delta (HD) enzyme-linked immunosorbent assay (ELISA) using a specific recombinant hepatitis delta antigen derived from a local dominant hepatitis delta virus (hepatitis D virus; HDV) strain in Taiwan has been established. The detection efficiency of this assay was comparable to that of the commercially available Abbott anti-HD radioimmunoassay (RIA) and could be useful in routine laboratory diagnoses of HDV infection.
AuthorsYung-Bin Kuo, Mei Chao, Yi-Hsuan Lee, Chau-Ting Yeh, Err-Cheng Chan
JournalClinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 19 Issue 5 Pg. 817-9 (May 2012) ISSN: 1556-679X [Electronic] United States
PMID22398247 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Hepatitis Antibodies
  • Hepatitis delta Antigens
  • Recombinant Proteins
Topics
  • Clinical Laboratory Techniques (methods)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Hepatitis Antibodies (blood)
  • Hepatitis D (diagnosis)
  • Hepatitis Delta Virus (immunology)
  • Hepatitis delta Antigens
  • Humans
  • Radioimmunoassay (methods)
  • Recombinant Proteins
  • Taiwan

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