A perivascular system releasing sirolimus prevented intimal hyperplasia in a rabbit model in a medium-term study.

Abstract

The main complication of aortocoronary reconstruction with vein grafts is restenosis in the course of time. The aim was to assess the effect of a periadventitial polyester mesh releasing sirolimus on intimal hyperplasia of autologous grafts. We implanted v. jugularis ext. into a. carotis communis in rabbits. The vein graft was either intact, or was wrapped with a pure polyester mesh, or with a sirolimus-releasing mesh. Three and six weeks after surgery, the veins were subjected to standard histological staining and the thicknesses of the tunica intima, the media and the intima-media complex were measured. Wrapping the vein with a mesh releasing sirolimus or with a pure mesh decreased the thickness of the intima in comparison with a vein graft by 73 ± 11% or 73 ± 8% after 3 weeks, and by 73 ± 9% or 59 ± 12% after 6 weeks, respectively. Sirolimus-releasing meshes reduced the thickness of the media by 65 ± 9% and 20 ± 12% after 3 and 6 weeks. The thickness of the intima-media complex in grafts with sirolimus-releasing meshes decreased by 60 ± 6% and 30 ± 13% in comparison with pure PES meshes, after 3 and 6 weeks, respectively. A periadventitial polyester mesh releasing sirolimus has the potential to become an effective device in preventing vein graft restenosis.

• Authors: Ivo Skalský, Ondrej Szárszoi, Elena Filová, Martin Pařízek, Andriy Lytvynets, Jana Malušková, Alena Lodererová, Eduard Brynda, Věra Lisá, Zuzana Burdíková, Martin Capek, Jan Pirk, Lucie Bačáková
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 427 Issue 2 Pg. 311-9 (May 10 2012) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 22387280 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Drug Implants
• Immunosuppressive Agents
• Polyesters
• Proliferating Cell Nuclear Antigen
• Sirolimus

Topics

• Animals
• Cell Count
• Cell Proliferation
• Chinchilla
• Drug Implants
• Graft Occlusion, Vascular (pathology, prevention & control)
• Hyperplasia (pathology)
• Immunohistochemistry
• Immunosuppressive Agents (administration & dosage, therapeutic use)
• Male
• Muscle, Smooth, Vascular (drug effects)
• Paraffin Embedding
• Polyesters
• Proliferating Cell Nuclear Antigen (metabolism)
• Rabbits
• Sirolimus (administration & dosage, therapeutic use)
• Surgical Mesh
• Tissue Fixation
• Tunica Intima (pathology)
• Tunica Media (pathology)
• Veins (transplantation)