Abstract |
Arsenic compounds have shown medical usefulness since they proved to be effective in causing complete remission of acute promyelocytic leukemia. In this work we obtained a fluorescently labeled arsenic compound that can be used with current fluorescence techniques for basic and applied research, focused on arsenic-induced apoptosis studies. This compound is an arsanilic acid bearing a covalently linked FITC that was chemically synthesized and characterized by fluorescence, UV-Vis, mass and FTIR spectrometry. In addition, we assessed its apoptotic activity as well as its fluorescent labeling properties in HL60 cell line as a leukemia cell model through flow cytometry. We obtained a compound with a 1:1 FITC: arsenic ratio and a 595 m/z, confirming its structure by FTIR. This compound proved to be useful at inducing apoptosis in the leukemia cell model and labeling this apoptotic cell population, in such a way that the highest FITC fluorescence correlated with the highest arsenic amount.
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Authors | A Lis Femia, C Facundo Temprana, M Silvia Amor, Mariano Grasselli, Silvia Del V Alonso |
Journal | Medicinal chemistry (Shariqah (United Arab Emirates))
(Med Chem)
Vol. 8
Issue 2
Pg. 222-9
(Mar 2012)
ISSN: 1875-6638 [Electronic] Netherlands |
PMID | 22385172
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Fluorescent Dyes
- Isothiocyanates
- isothiocyanic acid
- Arsanilic Acid
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Topics |
- Antineoplastic Agents
(chemical synthesis, chemistry, pharmacology)
- Apoptosis
(drug effects)
- Arsanilic Acid
(chemical synthesis, chemistry, pharmacology)
- Cell Separation
(methods)
- Drug Screening Assays, Antitumor
- Flow Cytometry
- Fluorescence
- Fluorescent Dyes
(analysis, chemical synthesis, chemistry)
- HL-60 Cells
- Humans
- Isothiocyanates
(chemistry)
- Molecular Structure
- Staining and Labeling
(methods)
- Structure-Activity Relationship
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