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Synthesis of a fluorescently labeled compound for the detection of arsenic-induced apoptotic HL60 cells.

Abstract
Arsenic compounds have shown medical usefulness since they proved to be effective in causing complete remission of acute promyelocytic leukemia. In this work we obtained a fluorescently labeled arsenic compound that can be used with current fluorescence techniques for basic and applied research, focused on arsenic-induced apoptosis studies. This compound is an arsanilic acid bearing a covalently linked FITC that was chemically synthesized and characterized by fluorescence, UV-Vis, mass and FTIR spectrometry. In addition, we assessed its apoptotic activity as well as its fluorescent labeling properties in HL60 cell line as a leukemia cell model through flow cytometry. We obtained a compound with a 1:1 FITC:arsenic ratio and a 595 m/z, confirming its structure by FTIR. This compound proved to be useful at inducing apoptosis in the leukemia cell model and labeling this apoptotic cell population, in such a way that the highest FITC fluorescence correlated with the highest arsenic amount.
AuthorsA Lis Femia, C Facundo Temprana, M Silvia Amor, Mariano Grasselli, Silvia Del V Alonso
JournalMedicinal chemistry (Shariqah (United Arab Emirates)) (Med Chem) Vol. 8 Issue 2 Pg. 222-9 (Mar 2012) ISSN: 1875-6638 [Electronic] Netherlands
PMID22385172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antineoplastic Agents
  • Fluorescent Dyes
  • Isothiocyanates
  • isothiocyanic acid
  • Arsanilic Acid
Topics
  • Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
  • Apoptosis (drug effects)
  • Arsanilic Acid (chemical synthesis, chemistry, pharmacology)
  • Cell Separation (methods)
  • Drug Screening Assays, Antitumor
  • Flow Cytometry
  • Fluorescence
  • Fluorescent Dyes (analysis, chemical synthesis, chemistry)
  • HL-60 Cells
  • Humans
  • Isothiocyanates (chemistry)
  • Molecular Structure
  • Staining and Labeling (methods)
  • Structure-Activity Relationship

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