Anticonvulsant and antioxidant effects of cyano-carvone and its action on acetylcholinesterase activity in mice hippocampus.

Abstract

The anticonvulsant effect of cyano-carvone, a monoterpene monocyclic, was investigated in epilepsy model induced by pilocarpine. Cyano-carvone at doses of 25, 50 or 75 mg/kg promoted a reduction of 16.7, 33 and 66.7%, respectively, against pilocarpine-induced seizures, and it was efficacious in increasing both the latency to first seizures and the survival percentage, resulting in 33.3, 67 and 91.7% of protection against death induced by seizures, respectively (P < 0.05). The reference drug atropine (25 mg/kg) also produced a significant protection (100%). Its monoterpene, at 25, 50 and 75 mg/kg, was also capable to increase the latency for installation of status epilepticus induced by pilocarpine, and presented a significant protection against lipid peroxidation and nitrite formation in mice hippocampus (P < 0.05). In addition, it was observed that the cyano-carvone pretreatment increased the acetylcholinesterase activity in mice hippocampus after pilocarpine-induced seizures. The present results clearly indicate the anticonvulsant ability of cyano-carvone, which can be, at least in part, explained by the increased activity of the acetylcholinesterase enzyme. Our data suggest that the action mechanism can also be due to a direct activation of the antioxidant enzymes that could be associated with a reduction observed in oxidative stress in mice hippocampus, probably involving an inhibition of free radical production.

• Authors: Dayane Alves Costa, Guilherme Antônio Lopes de Oliveira, Tamires Cardoso Lima, Pauline Sousa dos Santos, Damião Pergentino de Sousa, Rivelilson Mendes de Freitas
• Journal: Cellular and molecular neurobiology (Cell Mol Neurobiol) Vol. 32 Issue 4 Pg. 633-40 (May 2012) ISSN: 1573-6830 [Electronic] United States
• PMID: 22373695 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticonvulsants
• Antioxidants
• Monoterpenes
• Acetylcholinesterase

Topics

• Acetylcholinesterase (metabolism)
• Animals
• Anticonvulsants (pharmacology, therapeutic use)
• Antioxidants (pharmacology, therapeutic use)
• Disease Models, Animal
• Enzyme Activation (drug effects)
• Male
• Mice
• Monoterpenes (pharmacology, therapeutic use)
• Oxidative Stress (drug effects, physiology)
• Status Epilepticus (drug therapy, enzymology, metabolism)