Abstract |
5-Fluorouracil (5-FU) plays an important role in the chemotherapy of advanced gastric cancer. However, genetic factors that affect therapeutic efficacy of 5-FU warrant further investigation. In the present study, using stable transfection of the ezrin- radixin- moesin-binding phosphoprotein 50 (EBP50) gene, we explored the genetic influences on 5-FU-induced apoptosis of human gastric cancer cells. Stable overexpression of the EBP50 gene was determined by reverse transcription polymerase chain reaction (RT-PCR) assay and western blot analysis. After treatment with 5-FU, cell growth activities in vitro were investigated by MTT assay. Cell apoptosis was evaluated by Hoechst 33258 staining and flow cytometry of Annexin V-FITC/PI staining. Compared with the BGC823 or BGC823/neo cells, EBP50 mRNA and protein levels in the BGC823/EBP50 cells (EBP50-transfected BGC823 cells) were markedly higher. Chemosensitivity and apoptosis rates of the BGC823/EBP50 cells were higher compared to the BGC823 and BGC823/neo cells following treatment with 5-FU. Stable overexpression of extrinsic EBP50 distinctly increases the 5-FU-induced apoptosis of gastric cancer cells, and is a novel strategy by which to improve the chemosensitivity of gastric cancer to 5-FU.
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Authors | Xiao-Guang Lv, Meng-Yao Ji, Wei-Guo Dong, Xiao-Fei Lei, Meng Liu, Xu-Feng Guo, Jing Wang, Chuo Fang |
Journal | Molecular medicine reports
(Mol Med Rep)
Vol. 5
Issue 5
Pg. 1220-6
(May 2012)
ISSN: 1791-3004 [Electronic] Greece |
PMID | 22366766
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimetabolites, Antineoplastic
- Phosphoproteins
- Sodium-Hydrogen Exchangers
- bcl-2-Associated X Protein
- sodium-hydrogen exchanger regulatory factor
- Fluorouracil
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Topics |
- Antimetabolites, Antineoplastic
(pharmacology)
- Apoptosis
(drug effects, genetics)
- Cell Line, Tumor
- Drug Resistance, Neoplasm
(drug effects, genetics)
- Fluorouracil
(pharmacology)
- Humans
- Mitochondria
(genetics, metabolism, pathology)
- Phosphoproteins
(biosynthesis, genetics)
- Sodium-Hydrogen Exchangers
(biosynthesis, genetics)
- Stomach Neoplasms
(genetics, metabolism, therapy)
- Transfection
- bcl-2-Associated X Protein
(biosynthesis, genetics)
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