Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection.

Abstract

UNLABELLED:

Polygalacturonase-inhibiting proteins (PGIPs) are typically leucine-rich repeat (LRR) proteins that can inhibit the activity of fungal polygalacturonases (PGs). In this study, two new Ospgip genes, named Ospgip6 and Ospgip7 with consensus sequence of ten imperfect LRR motif located on rice chromosomes 8 and 9, were identified using BLAST analysis. Both of them appear to be extracellular glycoproteins. To have a global view of the dynamic gene expression pattern, seven Ospgip genes were first analyzed using the Affymetrix rice genome array data from online resource. All of these seven Ospgip genes showed variable expression patterns among tissues/organs. In order to further investigate the potential function of these Ospgip genes, the responses of Ospgip genes to the treatment of various phytohormones (abscisic acid, brassinosteroid, gibberellic acid, 3-indole acetic acid, jasmonic acid, kinetin, naphthalene acetic acid and salicylic acid) as well as fungal infection were analyzed by real-time PCR using time course array. Generally, all the Ospgip genes were slightly up-regulated in the indica rice cultivar Minghui 63 under GA(3), KT and NAA treatments (except Ospgip2, which was down-regulated under KT treatment). In the japonica rice cultivar Zhonghua 11, Ospgip genes were regulated by most treatments with the response time variability. We also analyzed putative cis-elements in the promoter regions of Ospgip genes. This dataset provided a versatile resource to understand the regulatory network of Ospgip genes during the process of phytohormones treatment and fungal infection in the model monocotyledonous plant, rice, and could aid in the transgenic breeding against rice fungal diseases.

KEY MESSAGE:

All the seven Ospgip genes showed variable expression patterns in Minghui 63 and their expressions were regulated by different phytohormone treatments or fungal infection in Minghui 63 and Zhonghua 11.

• Authors: Liaoxun Lu, Fei Zhou, Yong Zhou, Xiaolei Fan, Shuifeng Ye, Lei Wang, Hao Chen, Yongjun Lin
• Journal: Plant cell reports (Plant Cell Rep) Vol. 31 Issue 7 Pg. 1173-87 (Jul 2012) ISSN: 1432-203X [Electronic] Germany
• PMID: 22362377 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• PGIP protein, plant
• Plant Growth Regulators
• Plant Proteins

Topics

• Amino Acid Sequence
• Computational Biology
• Gene Expression Profiling
• Gene Expression Regulation, Plant
• Molecular Sequence Data
• Oligonucleotide Array Sequence Analysis
• Oryza (genetics, metabolism)
• Phylogeny
• Plant Growth Regulators (pharmacology)
• Plant Proteins (genetics, metabolism)
• Promoter Regions, Genetic
• Rhizoctonia (pathogenicity)
• Transcriptome