One of the type VI
intermediate filament proteins,
nestin, is expressed in neuroepithelial stem cells during neural embryogenesis.
Nestin is also expressed in a variety of
neoplasms. Its expression in
brain tumors has not been thoroughly studied. The objectives of this study were to survey
nestin expression in different types of
brain tumor, and to evaluate
nestin as a marker for diagnosis and prognosis. We used tissue microarrays of 257
brain tumors for an immunohistochemical overview of
nestin expression:
nestin was frequently expressed in
gliomas and
schwannomas. Most of the
gliomas that expressed high levels of
nestin were high-grade
gliomas (
anaplastic astrocytomas,
anaplastic oligodendrogliomas, anaplastic oligoastrocytomas, and
glioblastomas). We then focused on high-grade
gliomas and performed immunohistochemistry again, using whole-mount slides. As a result, we found (1) significantly different
nestin expression between
glioblastomas and other high-grade
gliomas, and (2) worse overall survival for high-grade
gliomas with high
nestin expression. Our results suggest that: (1)
nestin is a useful marker for diagnosis of high-grade
gliomas, (2)
nestin is helpful in diagnosis of
schwannomas, and (3)
nestin expression is related to poor prognosis in high-grade
gliomas.