Abstract |
We developed a novel near-infrared (NIR) fluorescent probe, GPU-167, for in vivo imaging of tumor hypoxia. GPU-167 comprises a tricarbocyanine dye as an NIR fluorophore and two 2-nitroimidazole moieties as exogenous hypoxia markers that undergo bioreductive activation and then selective entrapment in hypoxic cells. After treatment with GPU-167, tumor cells contained significantly higher levels of fluorescence in hypoxia than in normoxia. In vivo fluorescence imaging specifically detected GPU-167 in tumors 24 h after administration. Ex vivo analysis revealed that fluorescence showed a strong correlation with hypoxia inducible factor (HIF)-1 active hypoxic regions. These data suggest that GPU-167 is a promising in vivo optical imaging probe for tumor hypoxia.
|
Authors | Kensuke Okuda, Yasuyuki Okabe, Tetsuya Kadonosono, Takahiro Ueno, Bahaa G M Youssif, Shinae Kizaka-Kondoh, Hideko Nagasawa |
Journal | Bioconjugate chemistry
(Bioconjug Chem)
Vol. 23
Issue 3
Pg. 324-9
(Mar 21 2012)
ISSN: 1520-4812 [Electronic] United States |
PMID | 22335430
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Carbocyanines
- Fluorescent Dyes
- GPU-167
- Nitroimidazoles
|
Topics |
- Animals
- Carbocyanines
(chemistry)
- Cell Hypoxia
- Fluorescent Dyes
(chemistry)
- Mice
- Mice, Nude
- Neoplasms
(pathology)
- Nitroimidazoles
(chemistry)
- Spectroscopy, Near-Infrared
(methods)
|